Expression and Characterization of atrplHomolog from Rat
Biochemical and Biophysical Research CommunicationsPublished 1 November 1997
Klaus‐Dieter Preuss, Joachim K. Nöller, Elmar Krause, Alexandra Göbel, Irene Schulz
Citations37
SJR quartileQ2
SJR score0.75
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TL;DR
It is concluded that Rtrp3, a new member of the growing family of trp/trpl homologues in mammalians, is involved in "Ca(2+)-store-operated" Ca2+ entry into the cell.
Abstract
Mammalian homologues of the Drosophila trp/trpl-gene-family code for "Ca(2+)-store-operated" channels. Here we describe the cloning and expression of a trp/trpl homologous gene from rat brain. The clone is named Rtrp3 because of its high homology to the recently described Htrp3 (Zhu et al (1996) Cell 85:661-671). Expression of Rtrp3 in the mammalian COS-1 cell line leads to 100% increase of capacitative Ca2+ entry as compared to the controls. This capacitative calcium entry can be completely blocked with La3+ (10 microM). We conclude that Rtrp3, a new member of the growing family of trp/trpl homologues in mammalians, is involved in "Ca(2+)-store-operated" Ca2+ entry into the cell.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
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