Electrophysiologic and morphologic properties of neurons in dissociated chick spinal cord cell cultures
Developmental BiologyPublished 1 March 1974
Gerald D. Fischbach, Marc A. Dichter
Citations84
SJR quartileQ2
SJR score1.16
SNIP0.73
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Abstract
Neurons dissociated from embryonic chick spinal cords mature in relatively sparse cell culture and survive in vitro for several weeks. They generate action potentials and form both excitatory and inhibitory chemical synapses with one another. By electrophysiologic and morphologic criteria, it appears that the neuronal population (after 2–3 weeks) is made up of a variety of different cell types; few, if any, are motoneurons. Neuron cell bodies are not covered by glia or satellite cells and nerve processes are not myelinated. Thus, the cultures should permit more direct microelectrode and pharmacologic analysis of differentiation of cell specific properties and of synapse formation than is possible in the intact central nervous system.
Keywords
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