Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new ‘RING-finger’ motif
GenePublished 1 July 1993
Atsushi Ishida, Hidefumi Asano, Masayuki Hasegawa, Haruhiko Koseki, Takao Ono, Michihiro C. Yoshida
Citations51
SJR quartileQ2
SJR score0.68
SNIP0.72
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TL;DR
The human Mel-18 cDNA is isolated and characterized and the deduced hMel-18 protein contains 344 amino acids with a RING-finger motif, a helix-loop-helix (HLH)-like structure and a Pro/Ser-rich region.
Abstract
It has recently been reported that there exists a new 'RING-finger' protein family among the zinc-finger (Zf) proteins. Previously, we had isolated the mouse Mel-18 cDNA (mMel-18) encoding the nuclear RING-finger protein that exhibits an ability to bind to a nonspecific DNA column. Here, we have isolated and characterized the human Mel-18 cDNA (hMel-18) using the mMel-18 cDNA as a probe. The deduced hMel-18 protein contains 344 amino acids (38 kDa) with a RING-finger motif, a helix-loop-helix (HLH)-like structure and a Pro/Ser-rich region. The hMel-18 gene is conserved among vertebrates. Its mRNA is highly expressed in placenta, lung and kidney, but the level is low in liver, pancreas and skeletal muscle. Using in situ hybridization, we mapped hMel-18 to band q22 of chromosome 12. It is possible that the Mel-18/bmi-1 gene family represents a mammalian homologue of the Drosophila polycomb gene group.
Keywords
Biochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesDNA sequencing with chain-terminating inhibitors
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A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Analytical BiochemistryA technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
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A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
Nucleic Acids ResearchAn analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
5,144 Citations1987Marilyn Kozak
5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
Journal of Molecular BiologyAnalysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins
4,554 Citations1978J. Garnier, David J. Osguthorpe +1 more
The algorithm is shown to be at least as good as, and usually superior to, the reported prediction methods assessed in the same way and the implication in protein folding is discussed.
Annual Review of BiochemistryEmpirical Predictions of Protein Conformation
3,139 Citations1978Peter Y. Chou, Gerald D. Fasman
CellA new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins
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Two cDNAs were isolated whose dimerized products bind specifically to a DNA sequence, kappa E2, located in the immunoglobulin kappa chain enhancer, which plays a crucial role in both dimerization and DNA binding.
ScienceAmino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis
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The rapid degradation of injected alpha- and beta-casein as well as the inverse correlation of PEST regions with intracellular stability indicate that the presence of these regions can result in the rapid intrace cellular degradation of the proteins containing them.
Proceedings of the National Academy of SciencesBuffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
2,414 Citations1983Mark D. Biggin, Toby J. Gibson +1 more
The use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.
CellZinc fingers: Gilt by association
789 Citations1988Ronald M. Evans
Comparisons of cDNAs encoding potential finger proteins indicate the existence of at least two classes of proteins with specific nucleic acid recognition capabilities, characterized according to the number and position of the cysteine and histidine residues available for zinc coordination.
CellIdentification of cooperating oncogenes in Eμ-myc transgenic mice by provirus tagging
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The distribution of proviruses over the four common insertion sites suggests that provirus tagging can be used not only to identify the cooperating oncogenes but also to assign these genes to distinct complementation groups in tumorigenesis.
Nucleic Acids ResearchImprovement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP
699 Citations1986Saeko Mizusawa, Susumu Nishimura +1 more
The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc7GTP) in place of dGTP was found to be very useful and use of dc7G TP is concluded to improve the dide oxygen chain termination process for DNA sequencing.
NatureA structural taxonomy of DNA-binding domains
617 Citations1991Stephen C. Harrison
The structures of several classes of DNA-binding domains reveal a variety of designs for recognizing a specific site on DNA.
Annual Review of BiochemistryDNA RECOGNITION BY PROTEINS WITH THE HELIX-TURN-HELIX MOTIF
585 Citations1990Stephen C. Harrison, Aneel K. Aggarwal
The HTH Motifs, Characteristics of ProteinlDNA Contacts, and Anchoring the HTH to DNA: Foundations of Recognition .
CellNovel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in Eμ-myc transgenic mice
513 Citations1991Ygal Haupt, Warren S. Alexander +3 more
Myc-induced lymphomagenesis can entail the concerted action of several genes, including the presumptive nuclear regulator bmi-1, which encodes a Cys/His metal-binding motif (C3HC4) that resembles those in several DNA-binding proteins and defines a new category of zinc finger gene.
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The time course, stability, and specificity of the protein-AUUUA interaction suggests the possibility that the formation of this complex may target susceptible mRNA for rapid cytoplasmic degradation.
Molecular and Cellular BiologyContext Effects and Inefficient Initiation at Non-AUG Codons in Eucaryotic Cell-Free Translation Systems
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Under prescribed conditions, the fidelity of initiation in extracts from animal as well as plant cells closely mimics what has been observed in vivo, and recognition of an AUG codon in a suboptimal context was higher when the adjacent downstream sequence was capable of assuming a hairpin structure than when the downstream region was unstructured.
Trends in GeneticsImprinting a determined state into the chromatin of Drosophila
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The Polycomb gene of Drosophila melanogaster is a member of a class of genes involved in the clonal transmission of the repressed state of bomeotic regulatory genes through development, and genetic evidence suggests that this mechanism of repression might be imprinted in the structure of the chromatin.
Nucleic Acids ResearchDouble stranded DNA sequencing as a choice for DNA sequencing
309 Citations1988Hong Zhang, Randy Scholl +2 more
A step-by-step protocol for the alkaline denaturation of template DNA, and recommendations for the sequencing reaction.
The FASEB JournalSuperfamily of steroid nuclear receptors: positive and negative regulators of gene expression
301 Citations1991Walter Wahli, Ernest Martinez
The similarity between all these hormone response enhancer elements, as well as between the receptors themselves, indicates a conserved general strategy for the hormonal control of transcription by steroids.
Genes & DevelopmentDefinition of an efficient synthetic poly(A) site.
298 Citations1989N C Levitt, Daniel Briggs +2 more
The results indicate that the splicing of intron 2 in the rabbit beta-globin gene occurs ahead of polyadenylation and have important implications for termination of transcription.
The EMBO JournalBTG1, a member of a new family of antiproliferative genes.
298 Citations1992Jean-Pierre Rouault, Ruth Rimokh +8 more
The cloning and sequencing of the human BTG1 cDNA are reported and it is postulated that these two genes are the first members of a new family of antiproliferative genes.
Proceedings of the National Academy of SciencesEffect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.
292 Citations1989Stanley Tabor, C C Richardson
The lack of discrimination against dideoxynucleoside triphosphates using T7 DNA polymerase and Mn2+ results in uniform terminations of DNA sequencing reactions, with the intensity of adjacent bands on polyacrylamide gels varying in most instances by less than 10%.
Molecular and Cellular BiologyContext effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems.
281 Citations1989M Kozak
Proceedings of the National Academy of SciencesZinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
275 Citations1991Bert L. Vallée, Jeff Coleman +1 more
Three distinct motifs of DNA-binding zinc proteins are recognized: (i) zinc fingers, (ii) zinc clusters, and (iii) zinc twists; the structural and functional significance of these different motifs in their binding to DNA and other proteins participating in the transmission of the genetic message will become apparent.
Trends in Biochemical SciencesA novel zinc finger coiled-coil domain in a family of nuclear proteins
261 Citations1992Bramham A. Reddy, Laurence D. Etkin +1 more
NatureSequence similarity between the mammalian bmi-1 proto-oncogene and the Drosophila regulatory genes Psc and Su(z)2
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The ability of the HSH domain to function as a module that promotes DNA binding through dimerization was further demonstrated by attaching it to the heterologous basic region of the c-Jun proto-oncogene product.
NatureDrosophila genes Posterior Sex Combs and Suppressor two of zeste encode proteins with homology to the murine bmi-1 oncogene
189 Citations1991Brian P. Brunk, Elisabeth C. Martin +1 more
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Journal of Biological ChemistryExpression of novel DNA-binding protein with zinc finger structure in various tumor cells.
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Immunostaining with antibodies raised against partial Mel-18 peptide sequences demonstrated nuclear localization of the gene product, and it was demonstrated that this protein has DNA-binding capacity, and the zinc finger is responsible for the DNA binding.
PubMedMultiple determinants of eukaryotic mRNA stability.
114 Citations1989Don W. Cleveland, Tim J. Yen
The presence of instability determinants, and not the absence of sequences that dictate stability, appears to be required for regulated mRNA degradation.
Genes & DevelopmentxUBF contains a novel dimerization domain essential for RNA polymerase I transcription.
83 Citations1991Brian McStay, Mark W. Frazier +1 more
The gap region of the Dimerization domain is dispensable for dimerization but is absolutely required for transcription, which suggests that the gap region has a critical function in transcription distinct from any effect on dimerized or DNA binding.
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At-rich seuences were common in the 3' non-coding regions of mRNA encoding certain lymphokines, cytokines and oncogenes, and the AT-rich regions were more highly conserved than the coding regions between.
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