CONJUGAL TRANSFER OF THE DRUG RESISTANCE PLASMID pAMβ IN THE LACTIC STREPTOCOCCI
FEMS Microbiology LettersPublished 1 January 1980
M. J. Gasson, F. L. Davies
Citations91
SJR quartileQ3
SJR score0.60
SNIP0.59
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Abstract
M.J. Gasson, F.L. Davies; Conjugal transfer of the drug resistance plasmid pAMβ in the lactic streptococci, FEMS Microbiology Letters, Volume 7, Issue 1, 1
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A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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roximately 70% of the covalently closed circular DNA isolated from transformant cultures by dye buoyant density gradients was shown to be hybridizable to beta plasmid DNA.
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Evidence is presented indicating that the transfer of the beta plasmid is mediated by a mechanism that requires cell-to-cell contact, and this has been confirmed by the isolation of beta Plasmid from transconjugant clones and by their ability to then serve as donors of the erythromycin and lincomycin resistance markers.
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