Neuronal Properties of Clonal Hybrid Cell Lines Derived from Central Cholinergic Neurons
SciencePublished 5 December 1986
David N. Hammond, Bruce H. Wainer, James H. Tonsgard, Alfred Heller
Citations106
SJR quartileQ1
SJR score10.42
SNIP6.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Nine clonal hybrid cell lines have been developed from the septal region of the mouse basal forebrain and express characteristics typical of cholinergic neurons--choline acetyltransferase activity and immunore activity, neurite formation with neurofilament protein immunoreactivity, and aggregation in rotation-mediated cell culture.
Abstract
Clonal cell lines derived from specific types of central neurons can be used to identify and characterize properties specific to those neurons. With somatic cell fusion techniques, nine clonal hybrid cell lines have been developed from the septal region of the mouse basal forebrain. Two lines express characteristics typical of cholinergic neurons--choline acetyltransferase activity and immunoreactivity, neurite formation with neurofilament protein immunoreactivity, and aggregation in rotation-mediated cell culture. These cell lines may be useful for studying the trophic interactions that support the development and maintenance of central cholinergic connections.
Keywords
Neuroscience
Analytical BiochemistryA Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
225,378 Citations1976Mark A. Bradford
Analytical BiochemistryA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
211,158 Citations1976Marion M. Bradford
ScienceThe Cholinergic Hypothesis of Geriatric Memory Dysfunction
5,552 Citations1982Raymond T. Bartus, Reginald L. Dean +2 more
Biochemical, electrophysiological, and pharmacological evidence supporting a role for cholinergic dysfunction in age-related memory disturbances is critically reviewed and an attempt has been made to identify pseudoissues, resolve certain controversies, and clarify misconceptions that have occurred in the literature.
ScienceAlzheimer's Disease: A Disorder of Cortical Cholinergic Innervation
3,064 Citations1983Joseph T. Coyle, Donald L. Price +1 more
Advances in neurotransmitter systems involved in the symptomatic manifestations of neurological and psychiatric disorders reflect a close interaction between experimental and clinical neuroscientists in which information derived from basic neurobiology is rapidly utilized to analyze disorders of the human brain.
The Journal of Comparative NeurologyDistribution of cholinergic neurons in rat brain: Demonstrated by the immunocytochemical localization of choline acetyltransferase
967 Citations1983David M. Armstrong, Clifford B. Saper +3 more
The correspondence of the distribution of ChAT‐labeled neurons identified by the methods to earlier immunocytochemical and acetylcholinesterase histochemical studies and to connectional studies of these groups argues for the specificity of the ChAT antibody used.
The EMBO JournalLevels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation.
943 Citations1985Sigrun I. Korsching, Georg Auburger +3 more
Results support the concept that NGF acts as trophic factor for magnocellular cholinergic neurons of the basal forebrain, and mRNANGF was found in the hippocampus and cortex but not in the septum.
NeuroscienceCortical projections arising from the basal forebrain: A study of cholinergic and noncholinergic components employing combined retrograde tracing and immunohistochemical localization of choline acetyltransferase
745 Citations1984David B. Rye, Bruce H. Wainer +3 more
The neurochemical identity of ascending putative cholinergic pathways from the rat basal forebrain was investigated employing a method for simultaneously visualizing choline acetyltransferase immunoreactivity and retrogradely transported horseradish peroxidase-conjugated wheatgerm agglutinin.
Annals of NeurologyA unifying hypothesis for the cause of amyotrophic lateral sclerosis, parkinsonism, and alzheimer disease
700 Citations1981Stanley H. Appel
It is presented that each of these disorders is due to lack of a disorder‐specific neurotrophic hormone, and central nervous system tissue culture provides a convenient system in which to assay these neurotrophic hormones and should permit a test of the hypothesis.
Proceedings of the National Academy of SciencesNeurotransmitter Synthesis by Neuroblastoma Clones
690 Citations1972Takehiko Amano, Elliott Richelson +1 more
The results show that genes determining neurotransmitter species can be expressed in dividing cells, that the parental programs of gene expression are inherited, and that dividing cells can be programmed with respect to their ability to communicate with other cells.
NeuroscienceNerve growth factor increases choline acetyl-transferase but not survival or fiber outgrowth of cultured fetal septal cholinergic neurons
492 Citations1985Franz Hefti, Jukka Hartikka +4 more
Results indicate that acetylcholinesterase is a reliable marker for cholinergic cells in cultures of dissociated septal neurons, and nerve growth factor elevated the biochemically measured activity of choline acetyltransferase up to two-fold.
Proceedings of the National Academy of SciencesESTABLISHMENT OF FUNCTIONAL CLONAL LINES OF NEURONS FROM MOUSE NEUROBLASTOMA
486 Citations1969Gabriella Augusti‐Tocco, Gordon Sato
A striking morphological change was observed in the cells adapted to culture growth; they appeared as mature neurons, while the cells of the tumor appeared as immature neuroblasts.
Journal of Histochemistry & CytochemistryA modified histochemical technique to visualize acetylcholinesterase-containing axons.
437 Citations1985John C. Hedreen, Sarah J. Bacon +1 more
An improved histochemical method for light microscopic demonstration of acetylcholinesterase (AChE) has been developed and axonal staining, including arborizations, stands out against a clear background devoid of diffuse reaction product.
ChromosomaA system of nomenclature for band patterns of mouse chromosomes
366 Citations1973Muriel N. Nesbitt, Uta Francke
Mouse chromosomes banded by quinacrine mustard staining, by the ASG technique, or by Giemsa staining following trypsinization or chymotrypsinizing are described in detail.
CellA genetic analysis of extinction: Trans-dominant loci regulate expression of liver-specific traits in hepatoma hybrid cells
345 Citations1984Ann M. Killary, R. E. K. Fournier
It is reported here that the extinction of hepatic marker traits in these clones was strictly correlated with the retention of five particular fibroblast chromosomes, and a genetic locus, tissue-specific extinguisher-1 (Tse-1), which regulates hepatic TAT expression in trans is defined and localized.
Journal of Biological ChemistryThe structure, biochemical properties, and immunogenicity of neurofilament peripheral regions are determined by phosphorylation state.
324 Citations1985Martin J. Carden, William W. Schlaepfer +1 more
It is concluded that phosphorylation modifies the structure of the two larger neurofilament polypeptides along domains that are peripheral to the filamentous backbone and that these effects are more pronounced for NF200 than for NF150.
Neurochemistry InternationalCholinergic systems in mammalian brain identified with antibodies against choline acetyltransferase
287 Citations1984Bruce H. Wainer, Allan I. Levey +2 more
Of the monoclonal antibodies developed in the laboratory, one cross-reacts with enzyme derived from all mammalian species tested and has proven to be a very useful reagent for localization of ChAT immunoreactivity.
NeuroscienceCholine acetyltransferase-immunoreactive neurons intrinsic to rodent cortex and distinction from acetylcholinesterase-positive neurons
279 Citations1984Allan I. Levey, Bruce H. Wainer +3 more
It was concluded that cortical cholinergic cells are not visualized by acetylcholinesterase histochemistry, and are likely to be involved in local circuitry.
Journal of NeuroscienceMonoclonal antibodies to choline acetyltransferase: production, specificity, and immunohistochemistry
269 Citations1983Allan I. Levey, D.M. Armstrong +3 more
One of the antibodies was used to localize immunohistochemically known cholinergic structures in the rat brain, including motor neurons, basal forebrain neurons, and neostriatal neurons.
NatureSynthesis, storage and release of acetylcholine by a noradrenergic pheochromocytoma cell line
254 Citations1977Lloyd A. Greene, Glen Rein
It is shown here that noradrenergic cultures of PC12 cells can also synthesise, store and release acetylcholine.
Journal of HeredityTHE DEVELOPMENT OF SOME EXTERNAL FEATURES IN MOUSE EMBRYOS
231 Citations1943Hans Grüneberg
Proceedings of the National Academy of SciencesCholinergic metabolism and synapse formation by a rat nerve cell line.
224 Citations1977D Schubert, Steve Heinemann +1 more
The PC12 clone of a rat pheochromocytoma is able to synthesize acetylcholine, and the PC12 cell line is ability to form cholinergic synapses with a clonal cell line of skeletal muscle origin.
Biochemical GeneticsGenetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus)
218 Citations1969Robert J. DeLorenzo, Frank H. Ruddle
It has been demonstrated that the polymorphism of Gpi-1 is widely distributed in feral mice and was expressed in vitro and in four types of malignant tumors.
Journal of NeuroscienceExpression of neurofilament subunits in neurons of the central and peripheral nervous system: an immunohistochemical study with monoclonal antibodies
208 Citations1986JQ Trojanowski, N. Walkenstein +1 more
It is suggested that the expression of all three NF subunits is a common feature of CNS and PNS neurons and their processes, and of some cells of the diffuse neuroendocrine system.
Proceedings of the National Academy of SciencesNeuronal traits of clonal cell lines derived by fusion of dorsal root ganglia neurons with neuroblastoma cells.
199 Citations1985Doros Platika, Margaret H. Boulos +2 more
In an attempt to immortalize the gene products of single neurons, somatic cell hybrids were produced by fusion of embryonic rat dorsal root ganglion (DRG) neurons with mouse neuroblastoma cells, suggesting the selective expression of subsets of DRG neuronal genes.
NatureGrowth and differentiation of aggregating fetal brain cells in a serum-free defined medium
189 Citations1979Paul Honegger, Dominique Lenoir +1 more
This work describes the successful cultivation of aggregating fetal rat brain cells in a chemically defined, serum-free medium and applies this approach to differentiating primary cells of the central nervous system.
Developmental BiologyReconstruction of brain tissue from cell suspensions
180 Citations1972Beatrice B. Garber, A.A. Moscona
Coaggregation of suspensions of mouse cerebrum cells with chick cells from various brain areas, or from nonnervous tissues resulted in bispecific aggregates which were generally intermediate in size between typical aggregates of each of the component cell populations.
Proceedings of the National Academy of SciencesHurler's syndrome: biosynthesis of acid mucopolysaccharides in tissue culture.
170 Citations1966Reuben Matalon, Albert Dorfman
Proceedings of the National Academy of SciencesTarget neuron-specific process formation by embryonic mesencephalic dopamine neurons in vitro.
133 Citations1981Lisa M. Hemmendinger, Beatrice B. Garber +2 more
The formation of unique patterns of neuronal processes by dissociated neurons in vitro suggests that the information necessary for this differentiation is intrinsic to the dopamine neurons and their target cells.
Proceedings of the National Academy of SciencesMonoclonal antibodies recognize individual neurofilament triplet proteins.
119 Citations1982V Lee, H.‐L. Wu +1 more
The results strongly suggest that the NF subunits are distinct but related proteins and that the smaller NF subunit (P68 and P150) are not derived from larger NF proteins.
The Journal of Cell BiologyImmunofluorescence analysis of the time-course of extinction, reexpression, and activation of albumin production in rat hepatoma-mouse fibroblast heterokaryons and hybrids.
114 Citations1981Maryvonne Mével-Ninio, Mary C. Weiss
It is concluded that extinction is mediated by diffusible factor(s) from the L-cell parent that act in the hepatoma nucleus to prevent the formation of new albumin messenger RNA; reexpression and activation are gene dosage- dependent but extinction is not; and previously active genes are more rapidly expressed than previously silent ones.
NatureConservation of autosomal gene synteny groups in mouse and man
114 Citations1978P.A. Lalley, John D. Minna +1 more
The mouse gene map based on sexual genetics has been expanded by analysis of interspecific somatic cell hybrids segregating mouse chromosomes, so that the genetic maps of man and mouse can be compared.
Proceedings of the National Academy of SciencesNeurotrophic effects of hippocampal extracts on medial septal nucleus in vitro.
83 Citations1984Kosei Ojika, S. H. Appel
Soluble extracts from rat hippocampus enhance cholinergic activity of the rat medial septal nucleus cultured in serum-free defined medium, and varies with age, reaching a peak in 2- to 3-week-old rat hippocampus.
Proceedings of the National Academy of SciencesA general high-efficiency procedure for production of microcell hybrids.
83 Citations1981R. E. K. Fournier
An alternative micron nucleation protocol is reported, involving sequential treatment of the donor cells with Colcemid and cytochalasin B, which yielded micronucleated cells that hybridized with the same efficiency as whole cells.
Journal of Histochemistry & CytochemistryA comparison of eight different chromogen protocols for the demonstration of immunoreactive neurofilaments or glial filaments in rat cerebellum using the peroxidase-antiperoxidase method and monoclonal antibodies.
77 Citations1983John Q. Trojanowski, Maria Obrocka +1 more
The results obtained with both the anti-NF and anti-GF MA indicated that DAB with imidazole was the most sensitive chromogen protocol.
Somatic Cell and Molecular GeneticsChinese hamster � mouse hybrid cells segregating mouse chromosomes and isozymes
65 Citations1975John D. Minna, Thomas H. Marshall +1 more
This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.
Proceedings of the National Academy of SciencesNeuronal properties of hybrid neuroblastoma X sympathetic ganglion cells.
64 Citations1975Lloyd A. Greene, William Shain +5 more
One of the 37 hybrid cell lines obtained possesses high tyrosine 3-monooxygenase activity and synthesizes dopamine and can acquire neural properties not found with the parental neuroblastoma cells.
PubMedExpression of phenotypes in hybrid somatic cells derived from the nervous system.
55 Citations1975John D. Minna, J. Yavelow +1 more
The techniques of somatic cell hybridization allow a genetic analysis of differentiated functions of mammalian cells in vitro to yield hybrid progeny that retain human and segregate mouse chromosomes and isozymes.
NeuroscienceNeurotrophic effects of hippocampal target cells on developing septal cholinergic neurons in culture
33 Citations1987Jen-Chun Hsiang, Bruce H. Wainer +4 more
The influence of hippocampal target cells on the development of cholinergic septal neurons was studied in rotation-mediated reaggregating cell cultures to suggest that hippocampaltarget cells influence the development and survival of cholinesterase neurons.
Brain ResearchSelf-assembly of cortical plate cells in vitro within embronic mouse cerebral aggregates. Golgi and electron microscopic analysis
31 Citations1980Beatrice B. Garber, Peter R. Huttenlocher +1 more
Analysis of the events occurring during aggregate formation suggests that intrinsic cellular information accounts for the expression of basic pyramidal cell morphology, however, dendritic orientation and alignment are most likely determined by cell-cell interactions dependent upon specific cell surface recognition properties, as well as by geometric restraints imposed by the spherical or cylindrical shape of the aggregates.
Brain ResearchSelective association of embryonic murine mesencephalic dopamine neurons in vitro
28 Citations1981Lisa M. Hemmendinger, Beatrice B. Garber +2 more
The present study was undertaken to provide a quantitative description of the association of embryonic dopamine neurons in vitro and the effect of including neurons to which the dopamine neurons normally project (target cells) or cells from non-target areas was also studied.
Proceedings of the National Academy of SciencesClonal hybrid cell lines expressing cholinergic and adrenergic properties.
19 Citations1979Rolf Heumann, Mücella Öcalan +2 more
A "hybrid-hybrid" line containing cholinergic and adrenergic enzyme activities was produced, with the possibility of a simultaneous synthesis of noradrenaline and acetylcholine in single cells.
The EMBO JournalExpression of human neuronal antigens in a mouse neuroblastoma x human dorsal root ganglion cell hybrid.
8 Citations1983J.G. Dickson, Thomas P. Flanigan +1 more
This is the first demonstration of the continued expression of the continuing expression of a human species‐ and neuron‐specific gene product in a human‐mouse somatic cell hybrid.