Control of column influence on the wide range pH gradient in ion-exchange chromatography
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TL;DR
It is shown that programming of various wide range pH profiles which are not impaired by buffer interactions of the mobile phase with sorbent is possible and utilization of the wide range external pH gradient in cation-exchange chromatography of bovine haemoglobin is demonstrated.
Abstract
A method for optimization of the mobile phase composition in ion exchange chromatography with a wide range external pH gradient (approx. over 5 pH units) has been formulated. It is shown that programming of various wide range pH profiles which are not impaired by buffer interactions of the mobile phase with sorbent is possible. Utilization of the wide range external pH gradient in cation-exchange chromatography of bovine haemoglobin is also demonstrated.