Immunocytochemical evidence of Listeria, Escherichia coil, and Streptococcus antigens in Crohn's disease
GastroenterologyPublished 1 May 1995Open access
Ying Liu, Herbert J. Van Kruiningen, A. Brian West, Richard W. Cartun, Antoine Cortot, Jean‐Frédéric Colombel
Citations293
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TL;DR
The results suggest that Listeria spp.
Abstract
The results suggest that Listeria spp., E. coli, and streptococci, but not measles virus, play a role in the pathogenesis of Crohn's disease.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
Journal of the American Medical AssociationREGIONAL ILEITIS
1,508 Citations1932Burrill B. Crohn
A disease of the terminal ileum, affecting mainly young adults, characterized by a subacute or chronic necrotizing and cicatrizing inflammation, which frequently leads to stenosis of the lumen of the intestine, associated with the formation of multiple fistulas.
The LancetEffect of faecal stream diversion on recurrence of Crohn's disease in the neoterminal ileum
755 Citations1991P. Rutgeerts, Marc Peeters +6 more
The view that recurrence of Crohn's disease in the neoterminal ileum after curative ileal resection is dependent on faecal stream is strongly supported.
The LancetPATHOGENESIS OF CROHN'S DISEASE: MULTIFOCAL GASTROINTESTINAL INFARCTION
585 Citations1989A J Wakefield, Amar P. Dhillon +5 more
The findings suggest that Crohn's disease is mediated by multifocal gastrointestinal infarction, and this pathogenetic process is compatible with many of the clinical features of Crohn't disease, and its recognition has important implications for the identification of the primary cause of the illness and advances in clinical management.
The Histochemical JournalAminoalkylsilane-treated glass slides as support forin situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions
381 Citations1986Martin Rentrop, Bernhard Knapp +2 more
In situ hybridization was possible after a short immersion of the glass slides in a 2% solution of 3-aminopropyltriethoxysilane in acetone and it was found that especially harsh prehybridization drastically reduces the morphological integrity of the sections, thus rendering a reliable assignment of the label difficult.
GutMycobacterium paratuberculosis DNA in Crohn's disease tissue.
369 Citations1992Jeremy Sanderson, M. Moss +2 more
The presence in two thirds of Crohn's disease tissues but in less than 5% of ulcerative colitis tissues is consistent with an aetiological role for M paratuberculosis in Crohn't disease.
Proceedings of the National Academy of SciencesMolecular mimicry in virus infection: crossreaction of measles virus phosphoprotein or of herpes simplex virus protein with human intermediate filaments.
361 Citations1983Ryo Fujinami, Michael B. A. Oldstone +3 more
The results demonstrate that the phosphoprotein of measles virus and a protein of herpes simplex virus type 1 crossreact with an intermediate filament protein of human cells, and indicate that these monoclonal antibodies recognize different antigenic determinants on the intermediate filament molecule.
Histochemistry and Cell BiologyImproved section adhesion for immunocytochemistry using high molecular weight polymers of l-lysine as a slide coating
361 Citations1983Weimin Huang, S. J. Gibson +3 more
Poly-l-lysine has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining and it has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides.
Digestive Diseases and SciencesPossible role of mycobacteria in inflammatory bowel disease
334 Citations1984Rodrick J. Chiodini, Herbert J. Van Kruiningen +3 more
The findings raise the possibility that a previously unrecognized mycobacterium plays an etiologic role in at least some cases of Crohn's disease.
GastroenterologyGranulomatous Vasculitis in Crohn’s Disease
309 Citations1991Andrew J. Wakefield, Elizabeth A. Sankey +9 more
Vascular localization of granulomatous inflammation suggests that the intestinal microvasculature contains an early element in the pathogenesis of Crohn's disease.
Journal of Medical VirologyEvidence of persistent measles virus infection in Crohn's disease
250 Citations1993A J Wakefield, R. M. Pittilo +5 more
The observations suggest that measles virus is capable of causing persistent infection of the intestine and that Crohn's disease may be caused by a granulomatous vasculitis in response to this virus.
Digestive Diseases and SciencesPossible role of Mycobacteria in inflammatory bowel disease
227 Citations1984Walter R. Thayer, J A Coutu +3 more
An unclassifiedMycobacterium species has been isolated from two patients with Crohn's disease (CD), and although patients with positive PPD had elevated titers to this organism, the positive response of CD patients was not related to PPD responsiveness, area of involvement in the gut, nor to activity of the disease process.
British journal of surgeryIncidence of pathogenic bacteria from mesenteric lymph nodes and ileal serosa during Crohn's disease surgery
200 Citations1984N. S. Ambrose, Margaret M. Johnson +2 more
It is suggested that bacteria leak from the small bowel lumen in a high proportion of Crohn's disease patients, which may explain the pathogenesis of abscess and fistula in this disorder as well as the high rate of sepsis following elective surgery even in the absence of macroscopic contamination.
Journal of Medical VirologyDetection of herpesvirus DNA in the large intestine of patients with ulcerative colitis and Crohn's disease using the nested polymerase chain reaction
197 Citations1992A J Wakefield, Julie D. Fox +8 more
The high prevalence of coexistent HHV6 infection with either or both of these two viruses in ulcerative colitis tissue suggests that they may play a synergistic role in the pathogenesis of this disease.
GastroenterologyGranulomatous enterocolitis induced in rats by purified bacterial cell wall fragments
172 Citations1985R. Balfour Sartor, William J. Cromartie +2 more
It is concluded that bacterial cell wall fragments are capable of producing chronic granulomatous inflammation in the intestinal wall if present in appropriate particle size and concentration.
GutEscherichia coli antibodies in patients with inflammatory bowel disease.
165 Citations1978S. Tabaqchali, D. O’Donoghue +1 more
It is suggested that the presence of the high numbers of Escherichia coli antibodies is secondary to the disease process and is unlikely to be causally involved in the pathogenesis of the disease, but may play a role in the perpetuation of the Disease and in the extraintestinal complications.
American Journal of EpidemiologyPERINATAL RISK FACTORS FOR INFLAMMATORY BOWEL DISEASE: A CASE-CONTROL STUDY
164 Citations1990Anders Ekbom, Hans‐Olov Adami +3 more
Infants from families with low socioeconomic status had greater risk of inflammatory bowel disease than did infants fromfamilies with high socioeconomic status and perinatal health events independently increased the risk ofinflammatory bowel disease.
Infection and ImmunityCell-mediated immunity to intestinal infection
136 Citations1980Thomas T. MacDonald, P B Carter
Mice given a primary L. monocytogenes infection by the oral route were highly resistant to subsequent intravenous or oral challenge, and lymphocytes from the PP, MLN, and spleens of mice recovering from a primary oral infection were able to adoptively transfer immunity to normal recipients.
Infection and ImmunityMicrobial interference and colonization of the murine gastrointestinal tract by Listeria monocytogenes
122 Citations1979Z Zachar, Dwayne C. Savage
Journal of HistotechnologyAn Immunocytochemical Technique Offering Increased Sensitivity and Lowered Cost with a Streptavidin-Horseradish Peroxidase Conjugate
122 Citations1989Richard W. Cartun, Carl A. Pedersen
This work reports on a three step labeled avidinbiotin (LAB) immunoperoxidase that has been used successfully in the laboratory for the past three years and is easy to perform, accommodates busy technologists' schedules, and provides preparations with strong specific labeling and virtually no background interference.
PubMedPatients with active Crohn's disease have elevated serum antibodies to antigens of seven enteric bacterial pathogens.
110 Citations1984Martin J. Blaser, Richard A. Miller +2 more
Seroreactivity to enteric pathogens not resident in the bowel flora probably represents a nonspecific sensitization to cross-reacting antigens, and lack of response to the mycobacterial antigen suggests that widespread myCobacterial disease with high bacillary load is not present in Crohn's disease.
GastroenterologyA prospective study of viral and mycoplasma infections in chronic inflammatory bowel disease
108 Citations1990H. O. Kangro, Sonny K. F. Chong +3 more
Reactivation of latent herpesviruses was identified in 4 children with active disease and indicates that the converse relationship may also occur, and common pathogens were frequently associated with exacerbations and account for a large proportion of the commonly reported symptoms of a concurrent infection.
Journal of Clinical GastroenterologyThe “Early” Ulcerative Lesion of Crohnʼs Disease
108 Citations1980Robert R. Rickert, Harry W. Carter
The scanning electron microscope (SEM) was helpful in identifying the smallest of these lesions and was especially useful in defining a variety of villous abnormalities in the small bowel mucosa adjacent to the ulcers.
PubMedAn immunocytochemical search for infectious agents in Crohn's disease.
104 Citations1993Richard W. Cartun, Herbert J. Van Kruiningen +2 more
Results suggest that some of the granulomas in CD may result from immunologic processing of bacterial antigens following their penetration through a compromised mucosa, and E. coli and streptococcal antIGens may contribute to the pathogenesis of CD.
Digestive Diseases and SciencesExperimental disease in infant goats induced by aMycobacterium isolated from a patient with Crohn's disease
94 Citations1986Herbert J. Van Kruiningen, Rodrick J. Chiodini +4 more
Pilot studies were done to assess the pathogenicity of aMycobacterium which had been recovered from the diseased ileum of a patient with Crohn's disease, which represents an enteric pathogen capable of inducing granulomas of the distal small intestine of susceptible species.
GastroenterologyAn in-depth study of Crohn's disease in two French families
87 Citations1993Herbert J. Van Kruiningen, Jean–Frédéric Colombel +9 more
The circumstances and data suggest that an infectious microorganism is responsible for these clusterings of Crohn's disease in two French families.
The LancetINFANTILE GASTROENTERITIS ASSOCIATED WITH EXCRETION OF PESTIVIRUS ANTIGENS
67 Citations1989Robert H. Yolken, Flora Leister +4 more
The diarrhoeal disease in children excreting Pestivirus antigens resembled that in other children except that it was more commonly associated with signs and symptoms of respiratory inflammation.
The LancetCHLAMYDIA, CYTOMEGALOVIRUS, AND YERSINIA IN INFLAMMATORY BOWEL DISEASE
63 Citations1979E.T. Swarbrick, Howard Price +5 more
There was no evidence of chlamydial infection in Crohn's disease and no indication either that cytomegalovirus alters the outcome of acute attacks or that infection with Y. enterocolitica is being misdiagnosed as Crohn’s disease.
The LancetANTIBODIES AGAINST CHLAMYDIA OF LYMPHOGRANULOMA-VENEREUM TYPE IN CROHN'S DISEASE
49 Citations1979J.L. Schuller, I.V.D. Veeken +3 more
Antibodies against Chlamydia of lymphogranuloma-venereum type were detected in 38 (69%) of 55 patients with Crohn's disease and weak positive reactions were seen in only 2 or 21 patients with other gastrointestinal disorders and 1 of 50 healthy blood-donors.
Infection and ImmunityBacteroides vulgatus outer membrane antigens associated with carrageenan-induced colitis in guinea pigs
45 Citations1988James L. Breeling, A B Onderdonk +2 more
Antigens extractable from the bacterial outer membrane by EDTA are responsible for the enhancement of colitis inflammatory response in guinea pigs, and cross-absorbed antisera used in immunoblotting experiments identified four outer membrane proteins uniquely associated with the phenon capable of enhancing the colitis inflammation.
InfectionListeria isolations from feces of patients with diarrhea and from healthy food handlers
36 Citations1990H. E. Müller
The feces of patients were examined for well-documented enteropathogens such asCampylobacter, Salmonella, Shigella, Staphylococcus aureus, Yersinia enterocolitica, protozoa and rotavirus as well as for organisms of questionable enteropathogenic potency such as fungi.
PubMedStudies of Campylobacter jejuni in patients with inflammatory bowel disease.
34 Citations1984Martin J. Blaser, David Hoverson +4 more
Journal of Clinical MicrobiologyImmunoglobulin A (IgA) and IgG serum antibodies to mycobacterial antigens in Crohn's disease patients and their relatives
31 Citations1992L G Wayne, Daniel Hollander +4 more
Sera from patients with Crohn's disease, their relatives, their spouses, and unrelated healthy controls were assayed for immunoglobulin G (IgG) and IgA antibodies to Mycobacterium tuberculosis, M. avium, and M. gordonae, finding that the patients had significantly higher IgA responses to mycobacterial antigens than did either their relatives or the controls.
InfectionHistomorphology of experimental listeriosis
26 Citations1988B Heymer, Carl Heinz Wirsing von König +3 more
From the experimental models used and the studies performed much could be learned concerning the factors that determine the histomoraphological manifestations of listeriosis in humans.
GutChlamydiae and inflammatory bowel disease.
24 Citations1981Peter R. Elliott, T Forsey +3 more
No association was found between inflammatory bowel disease and infection with C. trachomatis or C. psittaci when patients were tested for the presence of these organisms using immunohistological, cell culture isolation, and serological techniques.
Australian and New Zealand Journal of MedicineHEPATIC INVOLVEMENT IN LISTERIOSIS
22 Citations1989Kurt Gebauer, Judith G. Hall +4 more
Sequential liver biopsies demonstrated the resolution of associated granulomas and microabscesses after the administration of high doses of penicillin for ten days in an adult with listeriosis.
PubMedRadioimmunologic measurements of naturally occurring bacterial antibodies. I. Human serum antibodies reactive with Escherichia coli in gastrointestinal and immunologic disorders.
22 Citations1973William R. Brown, Evelyn M. Lee
A radioimmunologic method for quantitative measurement of naturally occurring human serum antibodies reactive with Escherichia coli was developed and antibody concentrations were normal in many gastrointestinal conditions in which elevations might have been expected.
Microbial PathogenesisDeposition of circulating streptococcal lipoteichoic acid in mouse tissues
16 Citations1992J. Hyzy, Veronica M. Sciotti +2 more
Observations indicate that LTA, released by streptococci growing at remote body sites, can be carried by the blood to internal organs where it can accumulate and participate in pathogenesis.
Antonie van LeeuwenhoekLymphocytotoxic and microbial antibodies in Crohn's disease and matched controls
11 Citations1981Dieter W. Gump, E O Caul +8 more
Analysis of microbial antibody within the patient and control groups revealed a significant correlation between antibodies to the Pseudomonas-like bacterium and B. vulgatus, and there is no evidence of an increased incidence of infection with C. trachomatis, rotavirus or Norwalk virus in Crohn's disease.
