Anatomy of an identified serotonin neurone studied by means of injection of tritiated ‘transmitter’
NaturePublished 1 August 1974
V. W. Pentreath, G.A. Cottrell
Citations35
SJR quartileQ1
SJR score18.29
SNIP10.16
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
This neuronal system of the snail Helix pomatia offers a unique opportunity to study the proposed transmitter role of serotonin, and it is important to obtain direct information on the localisation and morphology of the presynaptic endings of the serotonin cell.
Abstract
THERE is an identifiable serotonin-containing neurone in each cerebral ganglion of the snail Helix pomatia1. Electrophysiological evidence suggests that each neurone sends a process into each cerebro–buccal connective2,3, and that monosynaptic connections are made in each buccal ganglion with other large identifiable neurones3. Because this neuronal system seems to offer a unique opportunity to study the proposed transmitter role of serotonin3,4, it is important to obtain direct information on the localisation and morphology of the presynaptic endings of the serotonin cell. This is not possible with dye5 or metal ion6 injection techniques, which do not seem to mark processes of the serotonin neurone at distances of more than a few millimetres from the neurone perikaryon.
Keywords
NeuroscienceEnvironmental Science
ScienceBranching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy
507 Citations1972Robert M. Pitman, Charles D. Tweedle +1 more
In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.
ScienceNeuronal Geometry: Determination with a Technique of Intracellular Dye Injection
415 Citations1968Antony O.W. Stretton, E. A. Kravitz
Repeated injection of the same cells in ganglia from different animals showed that cells have characteristic shapes and that the neuropil is highly structured.
Journal of NeurobiologyThe neuronal basis of behavior in <i>Tritonia</i>. I. Functional organization of the central nervous system
148 Citations1973A. O. Dennis Willows, D. A. Dorsett +1 more
An account of the brain (paired cerebral, pleural, and pedal ganglia) of the nudibranch mollusc Tritonia diomedia is presented and a standardized system of abbreviation and notation for the central ganglia, nerve trunks, and gaint somata is proposed.
The Journal of PhysiologySynaptic connexions of two symmetrically placed giant serotonin‐containing neurones
134 Citations1974G.A. Cottrell, James B. Macon
It is found that giant serotonin cell in Helix pomatia makes synaptic connexions with three non‐amine‐containing neurones: the anterior, middle and posterior buccal cells.
NatureSubcellular Localization of Serotonin in an Identified Serotonin-containing Neurone
121 Citations1970G.A. Cottrell, Neville N. Osborne
There is strong evidence that the amine acts as a transmitter on one particular type of neurone in the gastropod brain and the precise type of particle or particles associated with serotonin is not definitely known.
NatureDirect Postsynaptic Responses to Stimulation of Serotonin-containing Neurones
93 Citations1970G.A. Cottrell
There is one giant serotonin-containing neurone in each metacerebral ganglion of the slug Limax maximus and of the snail Helix pomatia and these cells can be identified histochemically and their content of serotonin can be measured by bioassay of individually dissected neurones.
The Journal of PhysiologyInput organization of two symmetrical giant cells in the snail brain
89 Citations1966E R Kandel, L Tauc
The ventral surface of the snail brain contains two symmetrical giant cells which are readily identifiable in each preparation and which make afferent and efferent connexions with the periphery and with the infraoesophageal ganglia.
Cell and Tissue ResearchAnatomy of giant serotonin-containing neurones in the cerebral ganglia of Helix pomatia and Limax maximus
61 Citations1973V. W. Pentreath, Neville N. Osborne +1 more
The Journal of Cell BiologyA COMMON SOURCE OF DIFFICULTY IN HIGH-RESOLUTION RADIOAUTOGRAPHY
57 Citations1969Lucien G. Caro
If the film does not gel rapidly or if electron microscopic examination reveals gross uneveness in the distribution of silver halide crystals, the time of cooling in the ice bath should be increased slightly and the schedule of melting and cooling may have to be modified accordingly.
ScienceProcion Brown: An Intracellular Dye for Light and Electron Microscopy
48 Citations1973Burgess N. Christensen
Procion browvn has been used to inject lamprey giant interneutros and imparts a pink color to the neuron in situ during iontophoresis, and has the advantage over cobaltolus chloride that postinjection treatment with ammonium sulfide is eliminated and the integrity of the surrounding neuropil is ensured.
Nature New BiologySelective Uptake of 5-Hydroxytryptamine by Axonal Processes in Helix pomatia
32 Citations1972V. W. Pentreath, G.A. Cottrell
ScienceNeuron Geometry and Circuitry via the Electron Microscope: Intracellular Staining with Osmiophilic Polymer
31 Citations1973Rhanor Gillette, Bruce Pomeranz
Intracellularly injected cobalt catalyzes the generation of an osmiumbinding polymer from 3,3'-diaminobenzidine in neurons of Aplysia californica, which permits detailed structural analysis of synaptology.
Single Cell Isotope Injection Technique, a Tool for Studying Axonal and Dendritic Transport
30 Citations1971Peter Schubert, Hans Dieter Lux +1 more
Radioactive precursors (amino acids and choline) were applied to cat spinal motoneurons by means of micro pipette electrophoresis and the synthesis of proteins within the nerve cell soma and their export into the axon was increased following antidromic stimulation.