Stress mediated changes in hypothalamic corticotropin releasing factor-like immunoreactivity
Life SciencesPublished 1 January 1987
Roberta L. Moldow, Abba J. Kastin, Markus Graf, Alan J. Fischman
Citations40
SJR quartileQ1
SJR score1.31
SNIP1.07
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The results suggest that the response of an organism to exposure to a long-term, high intensity stress involves both the release and synthesis of CRF-41, which is consistent with a release ofCRF from the hypothalamus during stress.
Abstract
Hypothalamic corticotropin releasing factor-like immunoreactivity (CRF-LI), plasma ACTH and corticosterone levels were measured by radioimmunoassay over a two hour period of restraint stress. The results of this study demonstrate a significant decrease in hypothalamic CRF-LI levels 15 and 30 minutes after the start of restraint stress which is followed by a significant increase at 60 minutes that is abolished by cycloheximide pretreatment. Plasma ACTH and corticosterone levels were significantly elevated after 15, 30, 60, 90, and 120 minutes of restraint stress. These results are consistent with a release of CRF from the hypothalamus during stress. The cycloheximide-sensitive increase in hypothalamic CRF-LI indicates that synthesis of CRF-41 occurs during prolonged stress. These results suggest that the response of an organism to exposure to a long-term, high intensity stress involves both the release and synthesis of CRF-41.
Keywords
MedicineNeuroscience
ScienceCharacterization of a 41-Residue Ovine Hypothalamic Peptide That Stimulates Secretion of Corticotropin and β-Endorphin
3,968 Citations1981Wylie Vale, Joachim Spiess +2 more
PeptidesThe distribution of corticotropin releasing factor-like immunoreactive neurons in rat brain
279 Citations1982John A. Olschowka, Thomas L. O’Donohue +2 more
The present findings suggest that a CRF-like peptide may be involved in a neurotransmitter or neuromodulator role, as well as a hypophysiotropic role.
EndocrinologyCorticosterone-Induced Changes in Hypothalamic Corticotropin-Releasing Factor (CRF) Content After Stress<sup>1</sup>
78 Citations1975Takenori Sato, Michiko Sato +2 more
Measurement of circulating ACTH levels in conjunction with CRF content after these stimuli have yielded sufficient information to assign mechanisms of altered synthesis and secretion to explain the observed changes inCRF content.
PeptidesHypothalamic CRF-like immunoreactivity in the rat after hypophysectomy or adrenalectomy
61 Citations1982Roberta L. Moldow, Alan J. Fischman
The increase in CRF-like immunoreactivity two weeks post-surgery is probably not related to direct feedback effects on release but may be due to increased synthesis secondary to long term removal of feedback inhibition.
PeptidesRadioimmunoassay of CRF-like material in rat hypothalamus
40 Citations1982Roberta L. Moldow, Alan J. Fischman
With this assay, CRF-like immunoreactivity which coeluted with ovine CRF on Sephadex G50 was detected in rat hypothalami and showed no crossreactivity with eight naturally occurring peptides.
NeuropeptidesRadioimmunoassay and chromatographic characterisation of CRF-41-like immunoreactivity in hypothalami from several species
36 Citations1982Elizabeth A. Linton, Philip J. Lowry
A sensitive and specific radioimmunoassay for the 41-residue ovine corticotropin releasing factor (CRF), employing direct radioiodination of the non-tyrosine containing synthetic peptide, detecting CRF-like immunoreactivity in ovine, bovine and rat stalk median eminence.
Regulatory PeptidesEffect of hypothalamus lesions on the presence of CRF-immunoreactive nerve terminals in the median eminence and on the pituitary-adrenal response to stress
36 Citations1982Fred J.H. Tilders, J. Schipper +2 more
The presence of CRFi nerve fibres in the median eminence is a prerequisite for rats to show a pituitary-adrenal response to ether stress and therefore represents the first functional evidence for the role of these hypothalamic CRFi-neurons.
EndocrinologyDynamic Changes in the Hypothalamic Content of Corticotropin Releasing Factor Following Noxious Stimuli: Delayed Response in Early Neonates in Comparison with Biphasic Response in Adult Rats
28 Citations1971Tsutomu Hiroshige, Takanori Sato +1 more
Dynamic changes in the hypothalamic content of corticotropin-releasing factor after stress were examined in the adult male rat as well as in the 2-day-old neonatal rat, showing a second peak value of CRF content at 80 minutes post stimulus.
PeptidesCircadian rhythm of corticotropin releasing factor-like immunoreactivity in rat hypothalamus
28 Citations1984Roberta L. Moldow, Alan J. Fischman
Levels of hypothalamic corticotropin releasing factor-like immunoreactivity (CRF-LI) were measured by radioimmunoassay (RIA) over a 24 hour light-dark cycle and found to exhibit two peaks and the trough between the two peaks was detected at 1700 hr which coincided with the peak in plasma corticosterone levels.
NeuroendocrinologyRat Hypothalamic Corticotropin-Releasing Factor (CRF) Content Remains Constant Despite Marked Acute or Chronic Changes in ACTH Secretion
18 Citations2008Naoki Yasuda, Monte A. Greer
It is concluded that hypothalamic CRF content maintains relative constancy under conditions of marked acute or chronic stimulation or suppression of ACTH secretion.
Annals of the New York Academy of SciencesASSAYS AND DYNAMICS OF CORTICOTROPIN‐RELEASING FACTOR ACTIVITY IN RAT HYPOTHALAMUS
13 Citations1977Tsutomu Hiroshige, Kenji Fujieda +2 more
It was shown that the two-peaked changes of CRF after ether-laparotomy stress in adult rats were composed of heterogeneous components, as revealed by differential effect of cycloheximide.
Life SciencesIn vivo potentiation of corticotropin releasing factor activity by vasopressin analogues
13 Citations1984Alan J. Fischman, Roberta L. Moldow
The ability of the neurohypophyseal hormones and related synthetic peptides to potentiate the action of synthetic ovine corticotropin releasing factor (CRF-41) was examined using male rats whose endogenous CRF release was blocked with chlorpromazine, morphine and nembutal.
European Journal of EndocrinologyCHANGES IN RAT HYPOTHALAMIC CONTENT OF CORTICOTROPHIN-RELEASING FACTOR (CRF) ACTIVITY, PLASMA ACTH AND CORTICOSTERONE UNDER STRESS AND THE EFFECT OF CYCLOHEXIMIDE
11 Citations1978Kenji Fujieda, Tsutomu Hiroshige
Hormone and Metabolic ResearchCircadian Rhythmicity of Hypothalamic CRH and its Central Nervous Regulation
7 Citations1970K. Retiene, F. Schulz
In contrast to the marked circadian rhythm of endocrine pituitary function only a slight fluctuation of hypothalamic CRH was observed in rats throughout the day, and it is proposed that a special "rhythm center" in the CNS acts in an inhibitory way on hypothalamic hormone synthesis.