The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes
CellPublished 1 May 1989
Keith R. Willison, Victoria Lewis, Kenneth S. Zuckerman, J L Cordell, C Dean, Karen Miller
Citations88
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
Analysis of the TCP-1 distribution in tissue culture cells showed that the polypeptide was associated with the cytoplasmic aspect of membranes of the trans-Golgi network (TGN), and the distribution in spermatids suggested that TCP- 1 was localized to structures often associated withThe developing acrosome.
Abstract
The t complex polypeptide 1 (TCP-1) is a protein of unknown function expressed in large amounts during spermatogenesis. Rat monoclonal antibodies recognizing TCP-1 have been prepared and used to immunoprecipitate and Western blot a 57 kd protein from germ cell and tissue culture cell extracts. In tissue culture cells, indirect immunofluorescent localization of antigen indicated a perinuclear distribution similar to that of the Golgi apparatus. Analysis of the TCP-1 distribution in tissue culture cells showed that the polypeptide was associated with the cytoplasmic aspect of membranes of the trans-Golgi network (TGN). The distribution in spermatids suggested that TCP-1 was localized to structures often associated with the developing acrosome. The TCP-1 antigenic epitopes are highly conserved, allowing the protein to be identified in cells across a wide variety of vertebrate species and tissues. These experiments suggest that TCP-1 may be essential for transport of proteins through the exocytic pathway in all cells and required in large amounts for acrosome formation in developing spermatids.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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