Radioimmunosorbent assay of allergens
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TL;DR
Theallergen assay method can conveniently be used to compare the "potency" of different allergen extracts and to check the extraction procedure, storage, and further treatment of allergens.
Abstract
Abstract A method has been developed for the assay of allergens and may be summarized as follows: 1. Filter paper discs are activated with BrCN. 2. The allergens are coupled to the reactive groups on the paper disc carrier. 3. IgE immunoglobulin, present in the sera of allergic patients, then attaches itself to the allergen coupled to the paper discs. 4. 125 I-labeled anti-IgE antibodies thereafter interact with the IgE molecules attached via the allergens to the paper carrier. Examples of the assay with the use of dog, cat, horse, cow, and timothy allergens are presented. The allergen assay method can conveniently be used to compare the potency of different allergen extracts and to check the extraction procedure, storage, and further treatment of allergens.