Formation of stable p53 homotetramers and multiples of tetramers
Molecular CarcinogenesisPublished 1 January 1992
Judith E. Stenger, Gregory A. Mayr, Kristine Mann, P Tegtmeyer
Citations107
SJR quartileQ2
SJR score0.95
SNIP0.64
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TL;DR
The composition of the tetramers was confirmed by partially cross‐linking p53 and dissociating tetramer into monomers, dimers, and trimers and fully cross‐linked p53 also behaves as tetrameric structures.
Abstract
We have used gradient gel electrophoresis and chemical cross-linking to analyze the quaternary structure of purified, wild-type, murine p53. Under nondenaturing conditions, p53 electrophoreses as tetramers and multiples of tetramers. Under denaturing conditions, fully cross-linked p53 also behaves as tetrameric structures. We confirmed the composition of the tetramers by partially cross-linking p53 and dissociating tetramers into monomers, dimers, and trimers.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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