The spontaneous release of acetylcholine from the denervated hemidiaphragm of the rat
The Journal of PhysiologyPublished 1 January 1963Open access
Jude F. Mitchell, Ann Silver
Citations178
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
It is shown that acetylcholine (ACh) is released from the isolated guinea-pig diaphragm in the absence of nerve stimulation and considered it possible that the resting release might represent the ACh responsible for the production of miniature end-plate potentials (m. p.p.s).
Keywords
Biochemistry, Genetics and Molecular Biology
The Journal of PhysiologyAn investigation of spontaneous activity at the neuromuscular junction of the rat
571 Citations1956A. W. Liley
The occurrence of spontaneous subthreshold activity at the neuromuscular junction of the albino rat has been observed and the characteristics of this activity have been investigated, both in isolated tissues and, to a less extent, in vivo.
The Journal of PhysiologyThe end‐plate potential in mammalian muscle
388 Citations1956I. A. Boyd, Andrew R. Martin
In the experiments to be reported here, the intracellular recording technique has been employed in a more complete investigation of transmission in mammalian skeletal muscle (cat), and was designed to determine whether the processes involved in neuromuscular transmission are identical in the two cases.
The Journal of PhysiologyThe effects of presynaptic polarization on the spontaneous activity at the mammalian neuromuscular junction
383 Citations1956A. W. Liley
In the present investigation a study has been made by intracellular recordings in the rat diaphragm of the relation between the frequency of the miniature discharge and electrotonic polarization of the motor nerve terminals.
The Journal of PhysiologyThe quantal components of the mammalian end‐plate potential
233 Citations1956A. W. Liley
Evidence is produced to show that the e.p. in the rat is also composed of summated miniature potentials and in the light of this finding a further investigation has been made into the phenomenon of facilitation at the mammalian neuromuscular junction.
The Journal of PhysiologyProperties of regenerating neuromuscular synapses in the frog
228 Citations1960Ricardo Miledi
Regeneration of nerve fibres into frog (R. temporaria) sartorius muscles was examined after either complete or partial denervation, and both sartorii and their nerves were dissected and mounted side-by-side for examination with intracellular micro-electrodes.
The Journal of PhysiologyCholine acetylase in antero‐ and retro‐grade degeneration of a cholinergic nerve
170 Citations1956Catherine O. Hebb, G. M. H. Waites
The problem is resolved by using a system in which acetyl CoA is provided separately and this method permits a direct measurement of ChA (choline acetylase) activity and is able to determine how the ChA of the cervical sympathetic nerve is affected by degenerative section.
The Journal of PhysiologySynthesis of acetylcholine by tissue of the central nervous system
151 Citations1945W. Feldberg
The Journal of PhysiologyThe action of botulinum toxin on motor‐nerve filaments
135 Citations1954V. B. Brooks
The Journal of PhysiologyThe release of acetylcholine in the isolated rat diaphragm
124 Citations1961K. Krnjević, Jude F. Mitchell
The Journal of PhysiologyDiffusion of acetylcholine in agar gels and in the isolated rat diaphragm
63 Citations1960K. Krnjević, Jude F. Mitchell
In the course of experiments in which acetylcholine released during nerve stimulation was collected from the isolated rat diaphragm, estimations were also made of the diffusion coefficient of ACh in gels of Ringer-Locke solution and agar.
Journal of Pharmacy and PharmacologyAssay of Acetylcholine on the Rat Blood Pressure
40 Citations1958Donald W. Straughan
Using sodium pentobarbitone with urethane as the anaesthetic and allowing the body temperature to fall to about 28" it has proved possible to get a long-surviving, stable sensitive preparation of rat blood-pressure preparation.
The Journal of PhysiologyAcetylcholine content of the rabbit plantaris muscle after denervation
39 Citations1962Catherine O. Hebb
Archives Internationales de Physiologie et de BiochimieThe Accessory Motor Innervation of the Diaphragm
18 Citations1961A. Rosenblueth, J Alanís +1 more
In cats, dogs, monkeys and rabbits, the section of the two phrenic nerves in the thorax does not lead to a paralysis of the diaphragm; inspiratory and expiratory discharges may be recorded.
The American Journal of SurgeryStudies on the phrenic nerves and the diaphragm of the dog
10 Citations1959Takumi Ogawa
The conclusion is reached that normally the phrenic nerves are the only nerves having trophic effects on the diaphragm, however, anastomosis of the distalphrenic nerve with the central end of another nerve (intercostal, recurrent laryngeal, vagus) is able to maintain a troPHic effect.
