Cytochrome <i>c</i> folding pathway: Kinetic native-state hydrogen exchange
Proceedings of the National Academy of SciencesPublished 26 August 2002Open access
Linh Hoang, Sabrina Bédard, Krishna M.G. Mallela, Yan Lin, S. Walter Englander
Citations116
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TL;DR
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each.
Abstract
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.
Keywords
Biochemistry, Genetics and Molecular BiologyPhysics and Astronomy
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