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pBluescript II: gene mapping vectors

Nucleic Acids ResearchPublished 1 January 1989Open access
Michelle A. Alting-Mees, Judith M. Short
Citations316
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TL;DR

The pBluescript 11 vectors, designed to facilitate rapid mapping of DNA inserts, include 21 unique restriction enzyme sites in the polylinker in either orientation relative to the f-gal promoter and single strand DNA rescue of either strand wih the aid of filamentous helper phage.

Keywords

Biochemistry, Genetics and Molecular Biology