An improved system for the assay of stimulation in mouse mixed leucocyte cultures
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TL;DR
Factors that affect the degree of stimulation in allogeneic cultures and those that contribute to the variability of the assay are defined.
Abstract
The addition of 2-mercaptoethanol to the medium used for the in vitro cultivation of mouse lymphoid cells has allowed the development of a sensitive and highly reproducible mixed leucocyte culture assay. In this system, the thymidine uptake by allogeneic cultures is 10 to 100-fold higher than that previously obtained with the same strain combination (CBA/J → C57B1/6J) and stimulation indices are 5 to 10-fold higher. This paper defines factors that affect the degree of stimulation in allogeneic cultures and those that contribute to the variability of the assay.