DYNAMIC RNA-RNA INTERACTIONS IN THE SPLICEOSOME
Annual Review of GeneticsPublished 1 December 1994
Hiten D. Madhani, Christine Guthrie
Citations357
SJR quartileQ1
SJR score6.46
SNIP2.51
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TL;DR
Recognition of the AG Dinucleotide at the 3' Splice Site and Base-Pairing with U2 snRNA Defines the Intron Branchpoint are described.
Abstract
Orthologs and paralogs are two fundamentally different types of homologous genes that evolved, respectively, by vertical descent from a single ancestral gene and by duplication. Orthology and paralogy are key concepts of evolutionary genomics. A ...Read More
Keywords
Biochemistry, Genetics and Molecular Biology
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Recognition of the UACUAAC box relies, at least in part, on Watson-Crick base pairing with the yeast U2 analogue, and allele-specific suppression of biological and biochemical phenotypes was observed.
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Excised group II introns in yeast mitochondria appear as covalently closed circles under the electron microscope and are branched and resemble the lariats arising through splicing of nuclear pre-mRNAs in yeast and higher eukaryotes.
NaturePRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome
337 Citations1991Beate Schwer, Christine Guthrie
The splicing factor PRP16 has been purified and shown to exhibit RNA-dependent ATPase activity, suggesting that it may be the prototype for a set of splicing factors which use ATP to drive a cycle of conformational changes.
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Cold Spring Harbor Monograph Archive13 Splicing of Precursors to mRNA by the Spliceosome
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285 Citations1993Cammie F. Lesser, Christine Guthrie
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In this chapter experiments are reviewed which led from the initial hypothesis about the role of U1 snRNP in pre-mRNA splicing to the elucidation of the function of all abundant nucleoplasmic snRNPs (Ul, U2, U4/6, and U5) in higher eukaryotes.
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A pathway of spliceosome assembly was deduced that has at least three stages and U2 small nuclear ribonucleoprotein alone binds to sequences of mRNA upstream of the 3' splice site.
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In both domains, the positions of base changes that block the second step of splicing correspond exactly to the site of insertion of pre-mRNA-type introns into the U6 gene of two yeast species, providing a possible explanation for the mechanism of how these introns originated and adding further evidence for the proposed catalytic role of U6 RNA.
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It is demonstrated that pairing between the universally conserved CU just downstream from the 5' junction interaction region and the 3' splice site AG contributes to efficient splicing of Schizosaccharomyces pombe introns that typify the AG-dependent class described in mammals.
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59 Citations1993Thorsten Wolff, Albrecht Bindereif
It is found that the 3' terminal loop of the singular U6 (nucleotides 65-69) is essential for initiating the U4-U6 base-pairing interaction, and this region provides evidence that the balanced stability of different conformations of U6 RNA is critical for its function.
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It is demonstrated for the mammalian splicing system that discrete positions within the ACAGAGA sequence and helix I of U6 snRNA function during the first and second step of splicing, suggesting that these two sequence elements are closely associated with the catalytic center of the spliceosome.
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50 Citations1993Pierre Legrain, C Chapon +1 more
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46 Citations1993Bertrand Séraphin, Stefanie Kandels‐Lewis
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40 Citations1994Hiten D. Madhani, C Guthrie
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