The participation of NADP, the transmembrane potential and the energy-linked NAD(P) transhydrogenase in the process of Ca2+ efflux from rat liver mitochondria
Archives of Biochemistry and BiophysicsPublished 1 January 1987
Anı́bal E. Vercesi
Citations65
SJR quartileQ1
SJR score0.91
SNIP0.78
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TL;DR
The pyridine nucleotide specificity, the participation of delta psi, and the energy-linked transhydrogenase in the process of Ca2+ efflux stimulated by the oxidized state of NAD(P) were examined in rat liver mitochondria energized by ascorbate + TMPD.
Abstract
The pyridine nucleotide specificity, the participation of delta psi, and the energy-linked transhydrogenase in the process of Ca2+ efflux stimulated by the oxidized state of NAD(P) were examined in rat liver mitochondria energized by ascorbate + TMPD. The following observations were made: The Ca2+ efflux rate is independent of the redox state of mitochondrial NAD, but is at a minimum when mitochondrial NADP is in the reduced state and accelerated several-fold when it is in the oxidized state. When the redox state of NADP is shifted to a more oxidized state, the steady-state level of Ca2+ in the medium increased and delta psi decreased in proportion to the mitochondrial NADP+ level. The activity of the energy-linked NAD(P) transhydrogenase seems to be a key element in determining the redox state of NADP and thus of Ca2+ retention and efflux from mitochondria.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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