Vitamin C in plasma: A comparative study of the vitamin stabilized with trichloroacetic acid or metaphosphoric acid and the effects of storage at − 70°, − 20°, 4°, and 25° on the stabilized vitamin

Abstract

Vitamin C concentration in human plasma was shown to be affected by storage time and temperature as well as by the kind of stabilizer used. Samples were stored for periods of time up to 21 days at temperatures of 25°, 4°, − 20°, and − 70°. Stabilizers employed in this study included trichloroacetic acid (TCA) and metaphosphoric acid (MPA). Plasma vitamin C demonstrated maximum stability in TCA or MPA when stored at − 70°. Other storage conditions tested yielded less accurate vitamin C values.