Guanine nucleotide-dependent carboxyl methylation of mammalian membrane proteins.
Journal of Biological ChemistryPublished 1 November 1988Open access
Peter S. Backlund, Robert R. Aksamit
Citations49
SJR quartileQ1
SJR score1.71
SNIP1.00
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TL;DR
The guanine nucleotide-dependent carboxyl methylations provide a possible mechanism for regulating the function of GTP-binding membrane proteins in the transduction of receptor-mediated signals of eukaryotic cells.
Abstract
A guanine nucleotide-dependent protein carboxyl methylation is demonstrated in mammalian cell membranes. The methylation of membrane proteins of Mr 20,000-23,000 requires S-adenosylmethionine, GTP or nonhydrolyzable GTP analogs, and a cytoplasmic methyltransferase. The protein methyl groups are stable at neutral pH and under basic conditions hydrolyze to produce methanol. The specific methyl acceptor proteins and methyltransferases varied between tissues and cell types, suggesting that these methylations have cell-specific functions. The guanine nucleotide-dependent carboxyl methylations provide a possible mechanism for regulating the function of GTP-binding membrane proteins in the transduction of receptor-mediated signals of eukaryotic cells.
Keywords
Biochemistry, Genetics and Molecular Biology
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