METABOLISM, TRANSFER AND STORAGE OF 5‐HYDROXYTRYPTAMINE IN BLOOD PLATELETS*
British Journal of Pharmacology and ChemotherapyPublished 1 January 1968Open access
A. Pletscher
Citations237
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Blood platelets, which contain substantial amounts of 5HT, can be studied in relatively physiological conditions in vitro-for example, in plasma or artificial buffer systems-and they also have the capacity to metabolize, transfer and store 5HT.
Keywords
ChemistryBiochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and Pharmaceutics
The Journal of PhysiologyStudies on the uptake of 5‐hydroxytryptamine by blood platelets
205 Citations1959G. V. R. Born, R. E. Gillson
NatureUltrastructural Localization of 5-Hydroxy-tryptamine in Blood Platelets
166 Citations1966J. P. Tranzer, M. Da Prada +1 more
The present communication shows that platelet 5HT is at least partially localized in special submicroscopic organelles which are not identical with α-granules.
British Journal of Pharmacology and ChemotherapyUPTAKE OF 5‐HYDROXYTRYPTAMINE BY PLATELETS
127 Citations1961R. S. Stacey
The antagonism of tryptamine was found to be competitive, and the possible site of its action is discussed, as well as a number of other substances on the uptake of 5-hydroxytryptamine by platelets.
PubMedThe mechanism of serotonin and catecholamine uptake by platelets.
123 Citations1959Hughes Fb, Brodie Bb
The Journal of PhysiologyAbsorption of serotonin (5‐hydroxytryptamine) and histamine by dog platelets
116 Citations1954John Humphrey, C. C. Toh
It is shown that platelets from dogs are capable of taking up serotonin from solution, and this finding raises the possibility that the serotonin found in platelets may be accumulated rather than formed in situ.
British Journal of Pharmacology and ChemotherapyTHE EFFECT OF IPRONIAZID AND IMIPRAMINE ON THE BLOOD PLATELET 5‐HYDROXYTRYPTAMINE LEVEL IN MAN
97 Citations1960Elizabeth F. Marshall, G. S. Stirling +2 more
The response was a fall to a level of one-sixth of the original in three weeks, with little change thereafter, in sharp contrast to the action of iproniazid which caused a rise of some 200% in the blood platelet 5-hydroxytryptamine level over the same period.
Journal of Pharmacology and Experimental TherapeuticsON THE MECHANISM OF ACTION OF RESERPINE: EFFECT OF RESERPINE ON CAPACITY OF TISSUES TO BIND SEROTONIN
81 Citations1957B.B. Brodie, E. G. Tomich +2 more
It is considered that the prolonged pharmacologic effect of reserpine is mediated by the persistent release of serotonin at synapses in the central autonomic nervous system.
NatureDecomposition of Dilute Hydrogen Peroxide in Alkaline Solutions
69 Citations1964Harold Maurice Cota, T. Katan +2 more
The Journal of PhysiologyThe uptake of 5‐hydroxytryptamine by blood platelets in the cold
57 Citations1959G. V. R. Born, Janet Bricknell
Experiments show that platelets take up 5-HT at 0-2° C, although much less than at 370 C, and indicate that the uptake in the cold is due to the diffusion of5-HT into the platelets, and that some 5- HT is adsorbed in or on the platelet.
British Journal of Pharmacology and ChemotherapyTHE RELATIONSHIP BETWEEN 5‐HYDROXYTRYPTAMINE AND ADENOSINE TRIPHOSPHATE IN BLOOD PLATELETS
54 Citations1958G. V. R. Born, G. I. C. Ingram +1 more
In patients receiving reserpine and in others with myeloid leukaemia, the amount of5-HT in the platelets and the uptake of 5-HT by them were depressed, while the amountof ATP was normal, suggesting the possibility that ATP is involved in the accumulation of 5,HT by platelets.
Life SciencesTwo sites of 5-hydroxytryptamine uptake in blood platelets
53 Citations1967A. Pletscher, W.P. Burkard +2 more
It is concluded that in guinea pigs the 5HT accumulation in the platelets occurs by two separate mechanisms, probably operating at the intracellular storage organelles and the cell membrane respectively, and the membrane transport seems to be linked to the energy metabolism of the platelet.
Cellular and Molecular Life SciencesDiminution of 5-hydroxytryptamine in thrombocytesin vitro by chlorpromazine and related compounds
52 Citations1961G. Bartholini, A. Pletscher +1 more
Cloropromazina, imipramina, cloroprotixene e amitriptilinain vitro diminiscono la 5-idrossitriptamina (5HT) endogena nei trombociti di coniglio e, per quanto si è visto, anche in quelli umani.
American Journal of Physiology-Legacy ContentBinding and Transport of Serotonin in Rabbit Blood Platelets and Action of Reserpine
46 Citations1958Isamu Sano, Yasuo Kakimoto +1 more
The mechanism of the transport and release are discussed and it is suggested that enzymatic processes may participate in both reactions.
NatureFormation of 5-Hydroxytryptophol from Endogenous 5-Hydroxytryptamine by Isolated Blood Platelets
44 Citations1964G. Bartholini, A. Pletscher +1 more
A neutral metabolite of 5HT could be demonstrated in the incubation medium of isolated platelets of the rabbit by paper and thin-layer chromatography and develops the typical green-yellow fluorescence of 5-hydroxyindolyl compounds in short-wave ultra-violet light if acidified with 4 N hydrochloric acid.
Journal of Pharmacology and Experimental TherapeuticsARALKYLAMINES WITH DIFFERENT EFFECTS ON THE METABOLISM OF AROMATIC MONOAMINES
42 Citations1966A. Pletscher, M. Da Prada +5 more
The different metabolic patterns of 5HT induced by the three Compounds in platelets and brain may be in part related to differences in their inhibitory effect on the oxidative deamination of the liberated 5HT.
Cellular and Molecular Life SciencesTwo types of 5-hydroxytryptamine release from isolated blood platelets
38 Citations1964G. Bartholini, A. Pletscher
Nach Einwirkung von Reserpin auf gewaschene, isolierte Thrombocyten werden neben 5-Hydroxytryptamin, 5- Hydroxyindolessigsäure und eine Substanz mit den Characteristika von N-Acetyl-5-hydroxyTryptamin im Inkubationsmedium (modifizierte Tyrodelösung) gefunden.
Cellular and Molecular Life SciencesIncrease of free 5-hydroxytryptamine in blood plasma by reserpine and a benzoquinolizine derivative
32 Citations1959M. K. Paasonen, A. Pletscher
Thrombosis and HaemostasisAn Electron Microscopic Study of Platelet Thrombus Formation in the Rabbit with Particular Regard to 5-Hydroxytryptamine Release
30 Citations1967Hans R. Baumgartner, J. P. Tranzer +1 more
Electron microscopic and histologic examination of rabbit ear vein segments 4 and 30 min after slight endothelial damage have yielded the following findings: platelets do not adhere to damaged endothelial cells and 5-hydroxytryptamine (5-HT) is released from platelets during viscous metamorphosis at least in part as 5-HT organelles.
Advances in pharmacologyElectron Microscopic Study of the Storage Site of 5-Hydroxytryptamine in Blood Platelets
29 Citations1968J. P. Tranzer, M DAPRADA +1 more
Combined ultrastructural, cytochemical, and biochemical observations indicate that the highly osmiophilic organelles present in platelets of various species contain 5-HT.
Biochemical PharmacologyEffect of monoamine liberators on the metabolism of 5-hydroxytryptamine in blood
29 Citations1965M. Da Prada, G. Bartholini +1 more
It is concluded that the previously described differences in the metabolic pattern of platelet 5HT after sympathomimetic amines and reserpine are probably due to an interference of the sympathomic amines with the oxidative deamination rather than with a localization of 5HT in two separate pools.
Life SciencesTwo types of monoamine liberation by chlorinated aralkylamines
28 Citations1965A. Pletscher, M. Da Prada +3 more
It is concluded that drug-induced differences in the rate of oxidative deamination of liberated 5HT might be responsible for the different metabolic pattern of the amine.
Journal of Pharmacology and Experimental TherapeuticsEFFECT OF DRUGS AND CATIONS ON ULTRASTRUCTURE AND 5-HYDROXYTRYPTAMINE CONTENT OF BLOOD PLATELETS
25 Citations1967M. Da Prada, J. P. Tranzer +1 more
It is concluded that reserpine, Ro 4-1284 and DL-amphetamine liberate 5-HT from platelets by a specific interference with special 5- HT-containing subcellular organdies in buffers deficient in Na or K.
International Journal of NeuropharmacologyOn the membrane effects of chlorpromazine: uptake of biologic amines by the blood platelet and red cell
25 Citations1964Z Fuks, Robert C. Lanman +1 more
The present study suggests that this view that chlorpromazine causes a decrease in the overall permeability of various biologic membranes is incorrect insofar as the permeability to organic amme compounds is concerned.
American Journal of Physiology-Legacy ContentAbsorption of Serotonin (5-Hydroxytryptamine) by Canine and Human Platelets
23 Citations1956Marjorie B. Zucker, Jennie Borrelli
The ability of the platelets to absorb serotonin could be correlated with their morphological appearance, whereas spiny spherical platelets often seen in shed blood did not, and the quantity of serotonin absorbed by washed dog platelets or by human platelets in platelet-rich plasma from unknown sources.
Cellular and Molecular Life SciencesFormation of 5-hydroxytryptophol by blood platelets after thrombin and reserpine
19 Citations1965M. Da Prada, G. Bartholini +1 more
Cellular and Molecular Life SciencesAction of reserpine and imipramine on intracellular storage of 5-hydroxytryptamine in blood platelets
18 Citations1967A. Pletscher, J. P. Tranzer
In isolierten Blutplättchen von Katzen vermindert Imipramin im Gegensatz zu Reserpin das endogene 5-Hydroxytryptamin (5HT) und die 5HT-Speicherorganellen nicht wesentlich, obwohl es wie Reser pin die Aufnahme von exogenem 5HT herabsetzt.
NatureInhibition of 5-Hydroxytryptamine Liberation from Blood Platelets by Reserpine
16 Citations1965G. Bartholini, M. Da Prada +1 more
The effect of reserpine on the metabolism of 5HT of isolated platelets in the absence and presence of glucose is investigated to investigate the mechanism behind the release of monoamines from various tissues including isolated blood platelets.
Elsevier eBooksMETABOLISM OF MONOAMINES BY BLOOD PLATELETS AND RELATION TO 5-HYDROXYTRYPTAMINE LIBERATION
13 Citations1966A. Pletscher, G. Bartholini +1 more
The metabolism of monoamines by blood platelets and relation to 5-hydroxytryptamine liberation is described and in the absence of major amounts of erythrocytes, the aldehyde is reduced to 7,000 times greater than that of 5-HydroxyTryptophol, whereas in the presence of eries, oxidation of the 5- Hydroxyindoleacetaldehyde to the corresponding acid takes place.
Journal of Molecular MedicineVerteilung und Beeinflussung von Metaboliten in franktionierten Homogenaten aus Blutplättchen
10 Citations1967E. Weber, H. Mondt
Life SciencesTemperature sensitivity of the passive outflow of 5-hydroxytryptamine from blood platelets
10 Citations1965M. Da Prada, A. Pletscher +1 more
The effect of temperature on the passive outflow of 5HT from isolated blood platelets of rabbits has been investigated under various experimental conditions and a marked temperature dependence could be demonstrated especially between 30° and 40°C.
Biochemical PharmacologyTemperature dependence of the passive in- and outflow of 5-hydroxytryptamine in blood platelets
9 Citations1966A. Pletscher, M. Da Prada +1 more
The 5HT liberation induced by sympathomimetic amines and reserpine shows a similar temperature sensitivity which is independent of the mechanism of action of the drugs of 5HT storage and may therefore be due to alterations in the passive outflow of liberated 5HT.
Biochemical PharmacologyAlterations of the 5-hydroxytryptamine outflux from blood platelets in vitro
6 Citations1965A. Pletscher, M. Da Prada +1 more
It is concluded that glucose, fructose, and ATP partly restore the storing capacity of the platelets for 5HT, whereas reserpine, Ro 4-1284, iproniazid and pargyline interfere with the outflux of the 5HT through platelet membranes.