Isozyme-selective stimulation of phospholipase C-β2 by G protein βγ-subunits
NaturePublished 1 December 1992
Montserrat Camps, Amanda Carozzi, Petra Schnabel, Alexander Scheer, Peter J. Parker, Peter Gierschik
Citations632
SJR quartileQ1
SJR score18.29
SNIP10.16
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TL;DR
PLC-β1 and P LC-β2 were stimulated by free βγ subunits and that PLC- β2 was the most sensitive to βγ stimulation, which indicates stimulation of PLC by βγSubunits is isozyme-selective and PLC -β2 is a prime target of βγ stimulated cells.
Abstract
Hydrolysis by phospholipase C (PLC) of phosphatidylinositol 4,5-bisphosphate is a key mechanism by which many extracellular signalling molecules regulate functions of their target cells. At least eight distinct isozymes of PLC are recognized in mammalian cells. Receptor-controlled PLC is often regulated by G proteins, which can be modified by pertussis toxin in some cells but not in others. In the latter cells, PLC-beta 1, but not PLC-gamma 1 or PLC-delta 1, may be activated by members of the alpha q-subfamily of the G protein alpha-subunits. An unidentified PLC in soluble fractions of cultured human HL-60 granulocytes is specifically stimulated by G protein beta gamma subunits purified from retina and brain. Identification of a second PLC-beta complementary DNA (PLC-beta 2) in an HL-60 cell cDNA library prompted us to investigate the effect of purified G protein beta gamma subunits on the activities of PLC-beta 1 and PLC-beta 2 transiently expressed in cultured mammalian cells. We report here that PLC-beta 1 and PLC-beta 2 were stimulated by free beta gamma subunits and that PLC-beta 2 was the most sensitive to beta gamma stimulation. Thus stimulation of PLC by beta gamma subunits is isozyme-selective and PLC-beta 2 is a prime target of beta gamma stimulation. Activation of PLC-beta 2 by beta gamma subunits may be an important mechanism by which pertussis toxin-sensitive G proteins stimulate PLC.
Keywords
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