Tumor necrosis factor induced DNA fragmentation of HL-60 cells
Biochemical and Biophysical Research CommunicationsPublished 1 December 1988
Laurence Elias, Penny B. Moore, Stephen M. Rose
Citations26
SJR quartileQ2
SJR score0.75
SNIP0.56
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TL;DR
It is possible that TNF-induced DNA fragmentation contributes to HL-60 differentiation and was blocked by inhibiting energy metabolism, but not by several inhibitors of cell surface signal transduction, protein or RNA synthesis, or free radical scavengers.
Abstract
Tumor necrosis factor (TNF) induces differentiation of HL-60 cells, with only slight effects upon proliferation and little or no cytotoxicity. TNF induced cytotoxicity of other target cell lines has been associated with DNA fragmentation. To assess whether TNF-induced DNA fragmentation might also contribute to HL-60 differentiation, studies were performed using a [3H]-dThd release assay. Between 1 and 2 hours of culture, significant [3H]-dThd release was induced by TNF at concentrations of 10 U/ml and greater. This response was blocked by inhibiting energy metabolism, but not by several inhibitors of cell surface signal transduction, protein or RNA synthesis, or free radical scavengers. DNA electrophoresis of the released DNA disclosed a wide range of low molecular weight fragments. It is possible that TNF-induced DNA fragmentation contributes to HL-60 differentiation.
Keywords
Immunology and MicrobiologyBiochemistry, Genetics and Molecular Biology
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