Identification of a Protein that Binds to the SH3 Region of Abl and Is Similar to Bcr and GAP-rho
SciencePublished 7 August 1992
Piera Cicchetti, Bruce J. Mayer, Gerald Thiel, David Baltimore
Citations552
SJR quartileQ1
SJR score10.42
SNIP6.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The 3BP-1 protein may be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Abstract
A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Keywords
MedicineAgricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
ScienceSH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
1,914 Citations1991Christine Koch, Deborah H. Anderson +3 more
Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
NatureFused transcript of abl and bcr genes in chronic myelogenous leukaemia
1,633 Citations1985Emma Shtivelman, Batia Lifshitz +2 more
Characterization of an 8-kilobase RNA specific to chronic myelogenous leukaemia shows it to be a fused transcript of the two genes, which is probably involved in the malignant process.
NaturePhosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases
763 Citations1990Christine Ellis, Michael F. Moran +2 more
It is shown that GAP, and two co-precipitating proteins of relative molecular masses 62,000 and 190,000 (p62 and p190) are phosphorylated on tyrosine in cells that have been transformed by cytoplasmic and receptor-like tyrosin kinases, which supports the idea that SH2 sequences direct the interactions of cytopLasmic proteins involved in signal transduction.
NatureA novel viral oncogene with structural similarity to phospholipase C
757 Citations1988Bruce J. Mayer, Michinari Hamaguchi +1 more
The previously uncharacterized avian sarcoma virus CT10 is cloned and its genome is sequenced, revealing a protein, p47gag-crk, that has blocks of sequence similarity to the amino-terminal, non-catalytic region of the non-receptor class of tyrosine kinases.
The Journal of Cell BiologyMicroinjection of recombinant p21rho induces rapid changes in cell morphology.
716 Citations1990Hugh F. Paterson, Annette J. Self +4 more
It is shown that microinjection of constitutively activated recombinant rho protein (Val14rho) into subconfluent cells induces dramatic changes in cell morphology: 15-30 min after injection cells adopt a distinct and novel phenotype with a contracted cell body and finger-like processes still adherent to the substratum.
NatureC. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains
602 Citations1992Scott G. Clark, Michael Stern +1 more
The results indicate that the sem-5 gene encodes a novel protein that functions in at least two distinct cell-signalling processes, both in vulval induction and in sex myoblast migration.
NatureBcr encodes a GTPase-activating protein for p21rac
477 Citations1991Dagmar Diekmann, Suzanne Brill +7 more
It is shown that the carboxy-terminal domains of the bcr-encoded protein (Bcr) and of a Bcr-related protein, n-chimaerin, are both GAP proteins for the Ras-related GTP-binding protein, p21rac, which suggests that Bcr could be a target for regulation by Rac and has important new implications for the role of bcr translocations in leukaemia.
CellMolecular cloning of cDNAs encoding the GAP-associated protein p190: Implications for a signaling pathway from ras to the nucleus
342 Citations1992Jeffrey Settleman, Vikram Narasimhan +2 more
Analysis of the predicted protein sequence reveals three distinct domains with homology to previously described sequences, raising the possibility that p190, acting via GAP, can transduce signals from p21ras to the nucleus, perhaps affecting expression of specific cellular genes.
NatureHomology of a yeast actin-binding protein to signal transduction proteins and myosin-I
314 Citations1990David G. Drubin, Jon Mulholland +2 more
The identification of SH3 domains in a family of otherwise unrelated proteins that associate with the membrane cytoskeleton indicates that this domain might serve to bring together signal transduction proteins and their targets or regulators, or both, in the membraneocytoskeleton.
ScienceNeuronal pp60 <sup> c- <i>src</i> </sup> Contains a Six-Amino Acid Insertion Relative to Its Non-Neuronal Counterpart
313 Citations1987Ricardo Martínez, Bernard Mathey-Prévôt +2 more
Brain c-src RNA contains an 18-nucleotide insertion at the position of the extra six amino acids within the NH2-terminal 16 kilodaltons of the molecule, which confirms that brain c- src RNA is encoded by a brain-specific messenger RNA.
The EMBO JournalN‐terminal mutations activate the leukemogenic potential of the myristoylated form of c‐abl.
284 Citations1989Peter K. Jackson, David Baltimore
The smallest of these deletions, delta XB, efficiently transforms lymphoid cells in vitro and causes leukemia in vivo demonstrating that gag sequences are not necessary for abl‐induced leukemogenesis.
CellAlternative 5′ exons in c-abl mRNA
280 Citations1986Yinon Ben‐Neriah, André Bernards +3 more
Four types of mouse c-abl cDNAs have been cloned from 70Z/3 lymphoid cells that have different 5' sequences encoding predicted N-terminal regions of 20-45 amino acids, which appears to be generated by alternative addition of 5' exon onto a common set of 3' exons.
Proceedings of the National Academy of SciencesThe noncatalytic src homology region 2 segment of abl tyrosine kinase binds to tyrosine-phosphorylated cellular proteins with high affinity.
225 Citations1991Bruce J. Mayer, Peter K. Jackson +1 more
It is demonstrated that an SH2 domain is sufficient to confer direct, high-affinity phosphotyrosine-dependent binding to proteins and suggest a general role for SH2 domains in cellular signaling pathways.
NatureA yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains
213 Citations1992Janet Chênevert, Kathleen Corrado +3 more
The isolation of two mutants defective specifically in cell polarization in response to peptide mating pheromones is described, which indicates that it may link the cytoskeleton to morphogenetic determinants on the cell surface.
The EMBO JournalDeletion of an N‐terminal regulatory domain of the c‐abl tyrosine kinase activates its oncogenic potential.
207 Citations1989Wolfgang-M. Franz, Paul Berger +1 more
The N‐terminal amino acids found to be necessary for the cellular inhibition of c‐abl tyrosine phosphorylation are part of a homologous region present in many non‐receptor tyrosin kinases, the v‐crk oncogene and phospholipase C‐II.
Journal of Molecular BiologyNovel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region gene
175 Citations1990Christine M Hall, Clinton Monfries +7 more
The similarity of two separate regions of n-chimaerin to domains of protein kinase C and BCR has intriguing implications with respect to its evolutionary origins, its function in the brain and potential phorbol-ester-binding properties.
Molecular and Cellular BiologyEffects of SH2 and SH3 deletions on the functional activities of wild-type and transforming variants of c-Src.
174 Citations1992Cynthia Seidel‐Dugan, Barbara E. Meyer +2 more
Molecular and Cellular BiologyAmino acid substitutions sufficient to convert the nontransforming p60c-src protein to a transforming protein.
169 Citations1986Jun‐ya Kato, Tatsuo Takeya +4 more
CellSequences of the A-MuLV protein needed for fibroblast and lymphoid cell transformation
165 Citations1983Ron Prywes, J. Gordon Foulkes +2 more
Systematic deletion of v-abl sequences showed that only 45,000 to the 130,000 molecular weight ofv-abl sequence in the A-MuLV protein is needed for fibroblast transformation and, at most, slightly more is neededFor lymphoid cell transformation.
Molecular and Cellular BiologySite-directed mutagenesis of the SH2- and SH3-coding domains of c-src produces varied phenotypes, including oncogenic activation of p60c-src.
150 Citations1990Hisamaru Hirai, Harold Varmus
PubMedMolecular cloning of sequence-specific DNA binding proteins using recognition site probes.
143 Citations1989Harinder Singh, Clerc Rg +1 more
Genes encoding sequence-specific DNA binding proteins can be isolated by screening lambda gt11 expression libraries with recognition site DNAs, derived from that developed for the isolation of genes using antibody probes.
PubMedActivation of pp60c-src transforming potential by mutations altering the structure of an amino terminal domain containing residues 90-95.
86 Citations1988W. M. Potts, A B Reynolds +2 more
It is reported that structural alterations within an amino terminal domain of pp60c-src, the exon 3 domain (residues 84-115) activate the oncogenic potential of the c-src gene product.
Journal of VirologyProtein stabilization explains the gag requirement for transformation of lymphoid cells by Abelson murine leukemia virus
55 Citations1985Ron Prywes, J Hoag +2 more
The specific instability of the p15-deleted protein in lymphoid cells explains the requirement of these sequences for lymphoid but not fibroblast transformation.
Current BiologyDefining a new GAP family
28 Citations1992Michael Fry
The identification of RasGAP as a putative target for Ras has prompted the search for distinct GAP activities among the other small GTP-binding proteins, and a brain-specific protein of unknown function, n-chimaerin, was also found to have significant homology with the RhoGAP peptide.