Brachyury downstream notochord differentiation in the ascidian embryo
Genes & DevelopmentPublished 15 June 1999Open access
Hiroki Takahashi, Kohji Hotta, Albert Erives, Anna Di Gregorio, R. W. Zeller, Michael Levine
Citations194
SJR quartileQ1
SJR score4.00
SNIP1.43
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TL;DR
It is shown that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform endoderm into notochord and encode a broad spectrum of divergent proteins associated withNotochord formation.
Abstract
The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform endoderm into notochord. Subtractive hybridization screens were conducted to identify potential Brachyury target genes that are induced upon Ci-Bra misexpression. Of 501 independent cDNA clones that were surveyed, 38 were specifically expressed in notochord cells. These potential Ci-Bra downstream genes appear to encode a broad spectrum of divergent proteins associated with notochord formation.
Keywords
Biochemistry, Genetics and Molecular Biology
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