The Oncogenic Cysteine-rich LIM domain protein Rbtn2 is essential for erythroid development
CellPublished 1 July 1994
Alan J. Warren, William H Colledge, Mark Carlton, Martin Evans, Andrew J.H. Smith, Terence H. Rabbitts
Citations617
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
In vitro differentiation of yolk sac tissue from homozygous mutant mice and sequentially targeted double-mutant ES cells demonstrates a block to erythroid development, which shows a pivotal role for a LIM domain protein in lineage specification during mammalian development and suggests that RBTN2 and GATA-1 are critical at similar stages of erystroid differentiation.
Abstract
The LIM domain protein rbtn2 is associated with T cell acute leukemias. We demonstrate that rbtn2 is a nuclear protein expressed in the erythroid lineage in vivo, and using homologous recombination, we show that it is essential for erythroid development in mice. The homozygous rbtn2 null mutation leads to failure of yolk sac erythropoiesis and embryonic lethality around E10.5. Moreover, in vitro differentiation of yolk sac tissue from homozygous mutant mice and sequentially targeted double-mutant ES cells demonstrates a block to erythroid development. This shows a pivotal role for a LIM domain protein in lineage specification during mammalian development and suggests that RBTN2 and GATA-1 are critical at similar stages of erythroid differentiation.
Keywords
Immunology and MicrobiologyMedicine
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