New Indicator of Histocompatibility Differences in vitro
Nature New BiologyPublished 1 October 1971
A. EKPAHA-MENSAH, J. C. Kennedy
Citations32
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TL;DR
The haemolytic plaque-forming cell response to sheep erythrocytes of cultures containing spleen cells from two different strains of mouse is presented to provide a sensitive indicator for detecting and assessing immune reactions between histoincompatible lymphoid cells and mitogenic factors released by such reactions.
Abstract
THE haemolytic plaque-forming cell (p.f.c.) response to sheep erythrocytes (SRBC) of cultures containing spleen cells from two different strains of mouse may be depressed if the mice differ sufficiently at the H-2 locus1. In our system, however, the usual result is stimulation rather than depression. We present evidence that such stimulation does not result, as has been suggested2,3, from synergism between two cell populations, but from an immune reaction based on histocompatibility differences between them. The system provides a sensitive indicator for detecting and assessing immune reactions between histoincompatible lymphoid cells and mitogenic factors released by such reactions.
Keywords
Immunology and MicrobiologyMedicine
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