Evidence for enkephalin immunoreactive neurons in the medulla oblongata projecting to the spinal cord
Neuroscience LettersPublished 1 September 1979
Tomas Hökfelt, Lars Terenius, H.G.J.M. Kuypers, Otto Dann
Citations302
SJR quartileQ2
SJR score0.77
SNIP0.67
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
In the present study a method is used, with which retrograde tracing with the fluorescent dye 'True Blue' is combined with the indirect immunofluorescence technique for visualizing enkephalin-like immunoreactivity. Evidence is presented for the existence of an enkephalin immunoreactive system originating in the lower medulla oblongata and projecting to the spinal cord.
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
NatureIdentification of two related pentapeptides from the brain with potent opiate agonist activity
3,786 Citations1975Joel W. Hughes, Terry Smith +4 more
The evidence is based on the determination of the amino acid sequence of natural enkephalin by the dansyl–Edman procedure and by mass spectrometry followed by synthesis and comparison of the natural and synthetic peptides.
American Journal of Clinical PathologyFluorescent Antibody Methods
1,567 Citations1969Wadi A. Bardawil, Benjamin L. Toy
ScienceSurgery in the Rat during Electrical Analgesia Induced by Focal Brain Stimulation
1,369 Citations1969David V. Reynolds
It was concluded that focal brain stimulation in this region can induce analgesia in the absence of diffusely applied "whole brain" stimulation.
Annals of NeurologyEndogenous pain control mechanisms: Review and hypothesis
1,241 Citations1978Allan I. Basbaum, Howard L. Fields
Evidence is presented that the intrinsic neural network that monitors and modulates the activity of pain‐transmitting neurons is mediated in part by endogenous opiatelike compounds (endorphins).
Proceedings of the National Academy of SciencesImmunohistochemical analysis of peptide pathways possibly related to pain and analgesia: enkephalin and substance P.
887 Citations1977Tomas Hökfelt, Å. Ljungdahl +3 more
The present immunohistochemical-anatomical findings support the hypothesis that stimulation-produced analgesia is related to activation of spinal and spinal trigeminal enkephalin interneurons forming axo-axonic synapses with (substance P?) pain afferents in the superficial laminae of the dorsal horn and the spinal trigEMinal nucleus.
ScienceRetrograde Axonal Transport in the Central Nervous System
859 Citations1972Jennifer H. LaVail, Matthew M. LaVail
This retrograde movement of protein from axon terminal to cell body suggests a possible mechanism by which neurons respond to their target areas.
The Journal of Comparative NeurologyImmunohistochemical localization of enkephalin in rat brain and spinal cord
818 Citations1978Madhabananda Sar, Walter E. Stumpf +3 more
The results of the histochemical staining show that certain structures which positively stain for enkephalin closely correspond to the distribution of opiate receptors in the brain and thus support the concept that the endogenous opiate peptides are involved in the perception of pain and analgesia.
NeuroscienceImmunohistochemical evidence of substance P-like immunoreactivity in some 5-hydroxytryptamine-containing neurons in the rat central nervous system
815 Citations1978Tomas Hökfelt, Å. Ljungdahl +6 more
Findings indicate that substance P and 5-HT may coexist not only in some cell bodies but also in axons and nerve endings in neurons of the lower medulla oblongata.
NeuroscienceImmunohistochemical studies using antibodies to leucine-enkephalin: Initial observations on the nervous system of the rat
774 Citations1976R. Elde, Tomas Hökfelt +2 more
Findings provide morphological support for the hypothesis that enkephalins are contained in nerve terminals close to opiate receptors as revealed by biochemical techniques.
PainCentral nervous system mechanisms of analgesia
717 Citations1976David J. Mayer, Donald D. Price
The intent of this review is to examine and synthesize the extensive progress that has been made in the last few years describing the anatomical, physiological and neurohumoral substrates of neural systems which modulate pain perception.
Neuroscience LettersThe distribution of enkephalin-immunoreactive cell bodies in the rat central nervous system
694 Citations1977Tomas Hökfelt, Robert Elde +3 more
With the indirect immunofluorescence technique the distribution of methionine-enkephalin-immunoreactive cell bodies was studied in the central nervous system of rats pretreated with colchicine.
Proceedings of the National Academy of SciencesNeurons containing beta-endorphin in rat brain exist separately from those containing enkephalin: immunocytochemical studies.
655 Citations1978F E Bloom, E Battenberg +3 more
Well-characterized antisera to porcine beta-endorphin were used to localize immunoreactive sites in cryostat sections of formaldehyde-fixed rat brain and are anatomically distinguishable from enkephalin-containing nerve cell and fiber pathways.
Proceedings of the National Academy of SciencesOpioid peptide enkephalin: immunohistochemical mapping in rat central nervous system.
622 Citations1977Rabi Simantov, M. J. Kuhar +2 more
In certain regions enkephalin immunofluorescence corresponds closely with the distribution of autoradiographic opiate receptor grains.
Neuroscience LettersRetrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat
605 Citations1975H.G.J.M. Kuypers, Vladimir A. Maisky
Horseradish peroxidase was injected in cervical, thoracic and lumbar segments of the cat's spinal cord through several transverse rows of narrowly spaced needle penetrations and transported retrogradely to brain stem neurons in the bulbar medial reticular formation, the vestibular complex and the red nucleus.
Brain ResearchAxonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve
419 Citations1971Krister Kristensson, Yngve Olsson +1 more
The results show that the hypoglossal neurones have the capacity to accumulate exogenous proteins after peripheral injection and this process must be a consequence of a fast retrograde axonal transport.
PainSerotonin-containing neurons: their possible role in pain and analgesia
399 Citations1977Rita B. Messing, Loy D. Lytle
Evidence suggests that increases in the activity of brain and spinal cord serotonin neurons are associated with analgesia and enhanced antinociceptive drug potency, whereas decreases in the activities of these neurons correlate with hyperalgesia and diminished analgesic drug potency.
Brain ResearchImmunohistochemical mapping of enkephalin containing cell bodies, fibers and nerve terminals in the brain stem of the rat
388 Citations1979George R. Uhl, Robert Goodman +3 more
Enkephalin immunoreactive perikarya, fibers and nerve terminals, visualized by the indirect immunohistofluorescent method in colchicine-pretreated animals, are localized in many discrete regions of the rat brain stem.
Neuroscience LettersRetrograde anoxal transport of fluorescent substances in the rat's forebrain
284 Citations1977H.G.J.M. Kuypers, Coriene E. Catsman‐Berrevoets +1 more
After double labelling of these neurons by means of Evans Blue either in combination with HRP or in conjunction with a DAPI-Primuline mixture, both labels could be demonstrated independently in the individual neurons.
Life SciencesImmunocytochemical localization of methionine enkephalin: Preliminary observations
234 Citations1977Stanley J. Watson, Huda Akil +2 more
The localization of met-enkephalin was carried out in the presence of excess leu-encephalin and yet could be blocked with equal amounts of meet-enkphalin.
HistochemieImmunohistochemical localization of three catecholamine synthesizing enzymes: aspects on methodology
219 Citations1972Tomas Hökfelt, Kjell Fuxé +2 more
It was found that cryostat sectioning followed by fixation in acetone or alcohol, a standard procedure in immunohistochemistry, was successful only with antibodies to the granule bound enzyme DBH, and the Vibratome ® technique may be a valuable tool for Immunohistochemical studies on the central nervous system.
Neuroscience LettersFluorescent retrograde neuronal labeling in rat by means of substances binding specifically to adeninethymine rich DNA
206 Citations1979Marina Bentivoglio, H.G.J.M. Kuypers +2 more
Six fluorescent substances, binding specifically to adenine-thymine rich DNA, were injected in rat caudate-putamen and resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe.
The Journal of Comparative NeurologyDifferential projections of cat medullary raphe neurons demonstrated by retrograde labelling following spinal cord lesions
179 Citations1978Richard Martin, Larry M. Jordan +1 more
Neurons of the medullary raphe nuclei in cats were retrogradely labelled following injection of horseradish peroxidase into the L6 spinal cord segment to determine the part of the cord through which raphe neurons project to the lumbar enlargement.
Neuroscience LettersThe projection from nucleus raphe magnus and other brainstem nuclei to the spinal cord in the rat: a study using the HRP blue-reaction
119 Citations1978George R. Leichnetz, L.R. Watkins +3 more
The direct NRM-spinal projection in the rat, involved in the modulation of pain transmission in the spinal cord dorsal horn, originates primarily from the expanded region of the nucleus in the rostralmost medulla.
Brain ResearchRetrograde peroxidase tracing of neurons combined with transmitter histochemistry
110 Citations1975A˚ke Ljungdahl, Tomas Ho¨kfelt +2 more
A technique by which pathways, retrogradely traced with peroxidase, in addition are characterized histochemically with regard to their neurotransmitter is described.
Life SciencesEffects of morphine, L-Dopa and tetrabenazine on the lamina V cells of spinal dorsal horn
45 Citations1975Hiroshi Takagi, Takayuki Doi +1 more
The hypothesis that the bulbospinal catecholaminergic neuron inhibits the pain transmission at the dorsal horn of the spinal cord and analgesic action of morphine is mainly mediated by activation of this neuron is supported.
Justus Liebig s Annalen der ChemieTrypanocide Diamidine mit vier Ringen in einem oder zwei Ringsystemen
19 Citations1973Otto Dann, Gerda Volz +5 more
