Fast Photochemical Oxidation of Proteins for Epitope Mapping
Analytical ChemistryPublished 6 September 2011Open access
Lisa M. Jones, Justin B. Sperry, James A. Carroll, Michael L. Gross
Citations120
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TL;DR
This study reports the use of footprinting via fast photochemical oxidation of proteins (FPOP) with OH radicals to characterize the epitope of the serine protease thrombin, the first time oxidative labeling has been used for epitope mapping.
Abstract
The growing use of monoclonal antibodies as therapeutics underscores the importance of epitope mapping as an essential step in characterizing antibody-antigen complexes. The use of protein footprinting coupled with mass spectrometry, which is emerging as a tool in structural biology, offers opportunities to map antibody-binding regions of antigens. We report here the use of footprinting via fast photochemical oxidation of proteins (FPOP) with OH radicals to characterize the epitope of the serine protease thrombin. The data correlate well with previously published results that determined the epitope of thrombin. This study marks the first time oxidative labeling has been used for epitope mapping.
Keywords
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