Purification of L-[3H] nicotine eliminates low affinity binding
Life SciencesPublished 1 January 1990
Elena Romm, Patrick M. Lippiello, Michael J. Marks, Allan C. Collins
Citations38
SJR quartileQ1
SJR score1.31
SNIP1.07
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Evidence presented here indicates that theLow affinity binding site is not stereospecific, is not inhibited by low concentrations of cholinergic agonists and is probably due to breakdown products of nicotine since purification of the L-[3H]nicotine eliminates the low affinity site.
Abstract
Some studies of L-[3H]nicotine binding to rodent and human brain tissue have detected two binding sites as evidenced by nonlinear Scatchard plots. Evidence presented here indicates that the low affinity binding site is not stereospecific, is not inhibited by low concentrations of cholinergic agonists and is probably due to breakdown products of nicotine since purification of the L-[3H]nicotine eliminates the low affinity site.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
ScienceStereospecific Nicotine Receptors on Rat Brain Membranes
320 Citations1980Carmelo Romano, Avram Goldstein
A stereospecific binding site for nicotine has been detected on rat brain membranes and competition studies with cholinergic agonists suggest that this site is a nicotinic Cholinergic receptor.
Molecular PharmacologyCharacterization of nicotine binding in mouse brain and comparison with the binding of alpha-bungarotoxin and quinuclidinyl benzilate.
310 Citations1982M J Marks, A C Collins
Differences in site densities, regional distribution, inhibitor potencies, and thermal denaturation indicated that nicotine binding was not the same as either QNB or alpha-BTX binding, and therefore that receptors for nicotine may represent a unique population of cholinergic receptors.
Molecular PharmacologyNicotinic binding sites in rat and mouse brain: comparison of acetylcholine, nicotine, and alpha-bungarotoxin.
265 Citations1986M J Marks, Jerry A. Stitzel +3 more
The results are consistent with the proposal that acetylcholine and nicotine bind to the same sites in both rat and mouse brain, whereas alpha-bungarotoxin binds to different sites.
The EMBO JournalGenes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
230 Citations1988Patrick Nef, Carole Oneyser +3 more
Four genes encode the related protein subunits that assemble to form the nicotinic acetylcholine receptor (nAChR) at the motor endplate of vertebrates and probably exists in all vertebrates since their counterparts have also been identified in the rat genome.
Journal of Biological Chemistryβ3: A New Member of Nicotinic Acetylcholine Receptor Gene Family Is Expressed in Brain
226 Citations1989Evan S. Deneris, Jim Boulter +3 more
In situ hybridization histochemistry, performed using probes derived from beta 3 cDNAs, demonstrated that the beta 3 gene is expressed in the brain, and proposed that thebeta 3 gene encodes a neuronal nAChR subunit.
Molecular PharmacologyThe binding of L-[3H]nicotine to a single class of high affinity sites in rat brain membranes.
124 Citations1986P M Lippiello, K G Fernandes
The present findings are consistent with the idea that there is a single class of high affinity nicotinic binding sites in rat brain with cholinoceptive properties.
Molecular Pharmacology[3H]acetylcholine and [3H](-)nicotine label the same recognition site in rat brain.
115 Citations1987Andrea M. Martino-Barrows, K J Kellar
Results indicate that [3H]acetylcholine and[3H](-)nicotine bind to the same nicotinic cholinergic recognition site in rat brain.
FEBS LettersBinding of optically pure (−)‐[<sup>3</sup>H]nicotine to rat brain membranes
46 Citations1983Leo G. Abood, Susan Grassi +1 more
A good correlation was observed between IC 50‐values and psychotropic potency of a series of N′‐alkyl substituted nicotine analogues and cholinergic drugs.
European Journal of PharmacologySubchronic treatment of rats with nicotine: Interconversion of nicotinic receptor subtypes in brain
39 Citations1988Luciana Romanelli, Birgitta Öhman +2 more
A significant increase in the number of cortical high-affinity (-)-[3H]nicotine binding sites was measured in rats treated with nicotine twice daily for 18 days and there was a significant decrease in the affinity of both subtypes of nicotinic binding sites.