Simultaneous radioimmunoassay of progestins, androgens and estrogens in rat testis
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TL;DR
A reliable method which permits the simultaneous measurement of pregnenolone, progesterone, 17-OH-progesterone, androstenedione, testosterone, dihydrotestosterone, estrone and estradiol in rat testis is described.
Abstract
Abstract A reliable method which permits the simultaneous measurement of pregnenolone, progesterone, 17-OH-progesterone, androstenedione, testosterone, dihydrotestosterone, estrone and estradiol in rat testis is described. After extraction of testicular homogenate with methanol, the method includes separation on a LH-20 column and steroid measurement by specific radioimmunoassays.
