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Simultaneous radioimmunoassay of progestins, androgens and estrogens in rat testis

Journal of Steroid BiochemistryPublished 1 February 1980
A. Bèlanger, S. Caron, Valérie Picard
Citations178

TL;DR

A reliable method which permits the simultaneous measurement of pregnenolone, progesterone, 17-OH-progesterone, androstenedione, testosterone, dihydrotestosterone, estrone and estradiol in rat testis is described.

Abstract

Abstract A reliable method which permits the simultaneous measurement of pregnenolone, progesterone, 17-OH-progesterone, androstenedione, testosterone, dihydrotestosterone, estrone and estradiol in rat testis is described. After extraction of testicular homogenate with methanol, the method includes separation on a LH-20 column and steroid measurement by specific radioimmunoassays.

Keywords

MedicineBiochemistry, Genetics and Molecular Biology