Recovery of a hybrid vector derived from bovine papilloma virus DNA pBR322 and the HSV tk gene by bacterial transformation with extrachromosomal DNA from transfected rodent cells
GenePublished 1 March 1983
Sekiguchi Takeshi, Takeharu Nishimoto, Kai Ryosuke, Mutsuo Sekiguchi
Citations11
SJR quartileQ2
SJR score0.68
SNIP0.72
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TL;DR
A bovine papilloma virus-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase gene is capable of transforming tk cells of mouse L and Syrian hamster BHK21 lines to tk + .
Abstract
A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase (tk) gene is capable of transforming tk- cells of mouse L and Syrian hamster BHK21 lines to tk+. In transformed cells a small proportion of the plasmid DNA is present as extrachromosomal elements while the remainder appears to be integrated into the genome. It was possible to recover plasmids indistinguishable from the input DNA by transformation of Escherichia coli bacteria with low-molecular-weight DNA isolated from the transformed mouse and hamster cells and consisting of BPV-pBR322-HSV tk+ plasmids.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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