Transformation of mammalian cells by avian myelocytomatosis virus and avian erythroblastosis virus
VirologyPublished 1 October 1979
Kristina Quade
Citations165
SJR quartileQ2
SJR score0.74
SNIP0.60
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TL;DR
Clones of rat cells transformed by avian myelocytomatosis virus strain MC29 and avian erythroblastosis virus have been isolated and display an altered morphology and are able to form colonies in soft agar.
Abstract
Clones of rat cells transformed by avian myelocytomatosis virus strain MC29 and avian erythroblastosis virus have been isolated. These cells display an altered morphology and are able to form colonies in soft agar. Focus-forming virus can be rescued from the transformed rat cells by fusion with chick cells.
Keywords
MedicineAgricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
VirologyDefectiveness of avian myelocytomatosis virus MC29: Isolation of long-term nonproducer cultures and analysis of virus-specific polypeptide synthesis
259 Citations1977Klaus Bister, Michael J. Hayman +1 more
Competition radioimmune assays demonstrated that protein extracts of NP MC29 cells contain inhibitory activity for precipitation of p19 and p27, but not for p15, and Pulse-chase experiments showed that the MC29–110K polypeptide turned over at a rate comparable to that of pr76, but none of the major structural proteins could be detected after the chase.
NatureTemperature-sensitive mutant of avian erythroblastosis virus suggests a block of differentiation as mechanism of leukaemogenesis
173 Citations1978Thomas Graf, Norbert Ade +1 more
A temperature sensitive mutant has been isolated from a replication defective acute leukaemia virus and in vivo, at 41 °C, the mutant shows a reduced leukaemogenic potential, indicating that the continuous expression of a viral gene product is necessary to maintain the undifferentiated state of the virus-transformedLeukaemia cells.
VirologyDefectiveness of avian erythroblastosis virus: synthesis of a 75K gag-related protein
158 Citations1979Michael J. Hayman, Brigitte Royer‐Pokora +1 more
Nonproducer clones of chicken fibroblasts or erythroblasts transformed by avian eryth-roblastosis virus of strains R and ES4 were isolated and a 75,000 molecular weight protein (AEV-75K) was isolated, shown to be antigenically related to the gag gene, but not to the pol or env genes.
Journal of VirologyIdentification of nucleotide sequences which may encode the oncogenic capacity of avian retrovirus MC29
154 Citations1978Diana Sheiness, Lois Fanshier +1 more
The genome of MC29 virus is characterized and nucleotide sequences that may encode the oncogenic potential of the virus are identified and lead to the conclusion that it may contain a previously unidentified gene(s) that is capable of transforming several distinct target cells.
VirologyEvidence for the multiple oncogenic potential of cloned leukemia virus: In vitro and in vivo studies with avian erythroblastosis virus
150 Citations1976Thomas Graf, Brigitte Royer‐Pokora +2 more
In vivo studies demonstrated that helper virus strains R and ES4 cause anemia and that they are probably responsible for the anemia-inducing capacity of AEV stocks.
VirologyTwo types of target cells for transformation with avian myelocytomatosis virus
127 Citations1973Thomas Graf
It is suggested that this type of cell is derived from the hematopoietic system and is the in vitro target cell for transformation with AMV and myeloblastosis associated virus.
VirologyA plaque assay for avian RNA tumor viruses
96 Citations1972Thomas Graf
It is confirmed that several strains of Rous sarcoma virus (RSV) contain associated viruses in excess and nontransforming plaque-forming viruses were found in varying proportions in stocks of clone-purified helper-independent strains of avian Sarcoma viruses.
Avian RNA Tumor Viruses
82 Citations1975Hidesaburô Hanafusa
Avian RNA tumor viruses are a group with certain characteristics that can be defined by both pathogenic and molecular criteria.
CellProperties of mammalian cells transformed by temperature-sensitive mutants of avian sarcoma virus
78 Citations1977Young C. Chen, Michael J. Hayman +1 more
Fibroblasts from European field vole and from normal rat kidney infected by avian sarcoma virus mutants which are temperature-sensitive for the maintenance of transformation can be transformed by wild-type avian Sarcomairus.
CellAvian myelocytomatosis and erythroblastosis viruses lack the transforming gene src of avian sarcoma viruses
63 Citations1978D. Stéhelin, Thomas Graf
Using labeled cDNA specific for the detection of the src gene of avian sarcoma viruses, it is found that avian myelocytomatosis virus strain MC29 and avian erythroblastosisirus strain ES4 lack nucleotide sequences related to thesrc gene.
CellVirogenic nontransformed cells isolated following infection of normal rat kidney cells with B77 strain rous sarcoma virus
46 Citations1974David Boettiger
The properties of this new class of avian RNA tumor virus-infected mammalian cell are described, which can be rescued by cell fusion with chicken cells, but they do not exhibit any evidence of viral transformation.
PubMedHeterogeneity of strain R avian (erythroblastosis) virus.
45 Citations1970R Ishizaki, T. Shimizu
Evidence suggesting heterogeneity of strain R derived directly from the chick was observed in the results of interference tests with Rous sarcoma virus (Rous-associated virus-2), which revealed the activity of an agent capable of infecting but not morphologically altering chick embryo cells.
Journal of VirologyMassive direct conversion of chick embryo cells by strain MC29 avian leukosis virus
45 Citations1967A J Langlois, Shankar Sankaran +2 more
This report describes the recently observed unique ability of strain MC29 leukosis virus to induce massive CEC culture infection with parallel morphological conversion within 72 hr under suitable conditions and compares CEC responses to strain R in comparison with those to another leukotic virus, strain R.
Journal of VirologyComplementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion
43 Citations1977Kathelyn S. Steimer, David Boettiger
Polyethylene glycol is effective as a fusing agent for the rescue of virus from Rous sarcoma virus-transformed mammalian cells and gave reproducible results in virus rescue experiments with an efficiency equal to the best Sendai virus preparations.
International Journal of CancerEffect of 3‐methylcholanthrene and dimethylnitrosamine on anchorage dependence of rat fibroblasts chronically infected with rauscher leukemia virus
36 Citations1973Nirmal K. Mishra, Wayne L. Ryan
No clear correlation between AD– transformation and the ability to grow in factor‐free medium was found, and the significance of these observations is discussed in relation to the “oncogene” hypothesis.
VirologyGenetic analysis of the defectiveness in strain MC29 avian leukosis virus
33 Citations1978Klaus Bister, Peter K. Vogt
It is concluded that avian myelocytomatosis virus MC29 is defective in all three genes essential for the replication of retroviruses, namely, env, pol, and gag.