Papillomavirus DNA replication
Journal of VirologyPublished 1 July 1991Open access
Paul F. Lambert
Citations124
SJR quartileQ1
SJR score1.28
SNIP0.90
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TL;DR
A review of new findings in papillomavirus replication presented in the context of earlier studies finds that both transand cis-acting viral elements are required for plasmid replication.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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449 Citations1991Mart Ustav, Arne Stenlund
It is shown that when produced from expression vectors in the absence of other viral gene products, the full length E2 transactivator polypeptide and a 72 kd polypePTide encoded by the E1 open reading frame in its entirety, are both necessary and sufficient for replication BPV in C127 cells.
ScienceTargeting the E1 Replication Protein to the Papillomavirus Origin of Replication by Complex Formation with the E2 Transactivator
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International Journal of CancerProperties of a non‐tumorigenic human cervical keratinocyte cell line
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A human cervical keratinocyte cell line, W12, has been initiated from a low‐grade cervical lesion histologically diagnosed as CIN I and an aneuploid karyotype has developed with progressive in vitro growth.
CellCharacterization of the Bovine Papilloma virus plasmid maintenance sequences
222 Citations1984Monika Lusky, Michael R. Botchan
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The bovine papillomavirus type 1 trans-acting replication factors were encoded by at least two distinct viral genes since the mutants fell into two complementation groups, rep and cop, and the mutations were recessive to the wild-type functions.
CellBovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle
135 Citations1987David M. Gilbert, Stanley N. Cohen
The results are consistent with a model in which BPV plasmids are chosen at random for replication throughout, and only during, S phase and support the view that the completion of S phase is a specifically activated event in the cell cycle rather than simply the end of one round of chromosomal DNA replication.
NatureA common function for polyoma virus large-T and papillomavirus E1 proteins?
128 Citations1984Philippe Clertant, Isabelle Seif
The observations suggest that the papillomavirus E1 proteins might have a function in common with the polyoma virus large-T proteins (which are required for the initiation of viral DNA replication), which are general characteristics of eukaryotic proteins involved in the control of DNA replication.
Journal of VirologyThe 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro
110 Citations1991Ira L. Blitz, Laimonis A. Laimins
The E1 open reading frame of bovine papillomavirus type 1 encodes factors necessary for extrachromosomal maintenance of the viral genome in transformed cells and full-length E1 protein was expressed in a baculovirus-insect cell system and found to be phosphorylated and localized to the nucleus of infected cells.
VirologyGenetic analysis of the 3′ early region transformation and replication functions of bovine papillomavirus type 1
103 Citations1986Dennis E. Groff, Wayne D. Lancaster
Trans-acting functions of the E2 and E5 ORFs in viral DNA replication and the E5 OrF in cell transformation are suggested, suggesting a requirement that the 3' half of E5 be intact for in vitro mouse cell transformation.
The EMBO JournalOrigin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells.
102 Citations1984Waldemar Waldeck, Frank Rösl +1 more
The origin of replication of bovine papilloma virus type 1 (BPV‐1) has been determined by isolating replicative intermediates (RI) of BPV‐transformed hamster embryo fibroblasts (HEF‐BPV) using electron microscopic techniques.
CellRepression of bovine papilloma virus replication is mediated by a virally encoded Trans-acting factor
98 Citations1986Leslie J. Berg, Monika Lusky +2 more
This work proposes a model that explains why the loss of E6 and E6/7 function leads to immunity of the infected cell, and defines a trans-acting negative modulator that regulates viral replication.
Proceedings of the National Academy of SciencesTransient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.
97 Citations1986Monika Lusky, Michael R. Botchan
It is shown that bovine papilloma virus type 1 early replication occurs faster than cellular DNA synthesis and the apparent requirement for a proximal enhancer function for replication may explain why certain BPV-1 constructions, when linked to bacterial plasmid sequences, can be maintained extrachromosomally while others cannot.
Journal of VirologyBovine papillomavirus type 1 3' early region transformation and plasmid maintenance functions
97 Citations1986M S Rabson, Carole Yee +2 more
Results obtained with deletion mutants and insertion mutants containing a linker with translational termination codons in all possible reading frames indicated that an E2 ORF gene product(s) is necessary for efficient transformation, as well as viral plasmid replication and maintenance in the context of the full BPV-1 genome.
CellNegative control of DNA replication in composite SV40-bovine papilloma virus plasmids
95 Citations1986James M. Roberts, Harold Weintraub
The controlled replication of SV-BPV composite replicons has enabled us to create permanent COS cell lines that stably maintain these plasmids as episomes and to show that replication control requires three BPV elements.
CellControl of plasmid replication—How do DNA iterons set the replication frequency?
94 Citations1990Kurt Nordström
Computer studies based on data about li indicate that the kinetics are essentially hyperbolic, and a con- trol curve is predicted that is significantly less steep than that of Lee and Bailey (Figure 1; Womble and Rownd, 1988a); this would give a slower adjustment of the copy number from deviations back to the steady-state value than ahyperbolic response would.
Proceedings of the National Academy of SciencesFormation of the complex of bovine papillomavirus E1 and E2 proteins is modulated by E2 phosphorylation and depends upon sequences within the carboxyl terminus of E1.
94 Citations1991Monika Lusky, E Fontane
The results suggest that the replication deficiency of some E1 mutants reflects their inability to associate with E2, and it is suggested that the phosphorylation state of E2 modulates the association of the two proteins.
Journal of VirologyIdentification of a 68-kilodalton nuclear ATP-binding phosphoprotein encoded by bovine papillomavirus type 1
88 Citations1990Shuo Sun, Lauren Thorner +3 more
It is shown by ATP affinity labeling that the E1 protein produced in COS cells binds ATP and that this activity is abolished by a point mutation which converts the codon for proline 434 to serine, suggesting that the ATP-binding activity of E1 is necessary for its putative role in viral DNA replication.
Proceedings of the National Academy of SciencesThe activation domain of the bovine papillomavirus E2 protein mediates association of DNA-bound dimers to form DNA loops.
77 Citations1991James D.R. Knight, Rui Li +1 more
The E2 transactivator protein of bovine papillomavirus binds its specific DNA target sequence as a dimer and the looping function of E2 maps to the 161-amino acid activation domain, which supports the looped model of transcription activation by enhancers.
Journal of VirologyReplication of bovine papillomavirus type 1 DNA initiates within an E2-responsive enhancer element
75 Citations1990Lijun Yang, Michael R. Botchan
The patterns detected show that bovine papillomavirus type 1 replicates in these cells bidirectionally and that one replication origin site in the viral genome is utilized.
Journal of VirologyProduction of human papillomavirus type 16 virions in a keratinocyte cell line
74 Citations1990Julia Nicole Sterling, Margaret Stanley +2 more
This experimental system appears to permit the completion of the HPV-16 life cycle in virus-containing keratinocytes.
Journal of VirologyPhenotypic analysis of bovine papillomavirus type 1 E2 repressor mutants
73 Citations1990Paul F. Lambert, Brian C. Monk +1 more
The phenotypes of BPV-1 mutants which are disrupted in their capacity to encode either the E2 transcriptional repressor or the E8/E2 fusion peptide are described and experiments are described which demonstrate that the E9/E1 gene product functions similarly to E2-TR.
Annual Review of GeneticsTHE GENETICS OF BOVINE PAPILLOMAVIRUS TYPE 1
69 Citations1988Paul F. Lambert, Carl C. Baker +1 more
Article de synthese sur les connaissances actuelles du papillomavirus de type 1 concernant l'expression genique, the transformation cellulaire, the replication de l'ADN viral and l' expression des genes tardifs.
Journal of VirologyOpen reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells
65 Citations1989K Neary, Daniel DiMaio
Results suggest that translation of both ORF E6 and the 3' end of ORf E7 is required for efficient induction of anchorage-independent growth by the intact BPV1 genome.
Molecular and Cellular BiologyComplementation of a Bovine Papilloma Virus Low-Copy-Number Mutant: Evidence for a Temporal Requirement of the Complementing Gene
58 Citations1986Leslie J. Berg, Karam B. Singh +1 more
These results, along with other findings, suggest that the establishment of BPV plasmid replication occurs in two stages: an initial amplification of the incoming DNA followed by stable homeostatic replication which maintains the existing copy number.
Journal of VirologyA bovine papillomavirus type 1-encoded modulator function is dispensable for transient viral replication but is required for establishment of the stable plasmid state
55 Citations1986Monika Lusky, Michael R. Botchan
It is reported here that expression of M, which is a repressor of transient BPV replication and is not required as a positive factor in these assays, is required for the establishment of the viral genome as a stable nuclear plasmid.
The EMBO JournalBovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation.
55 Citations1988Daniel DiMaio, J Settleman
The construction and characterization of a temperature‐sensitive papillomavirus mutant and strong genetic evidence that continued ORF E2 activity is required for maintenance of BPV1‐induced transformation and for normal viral DNA replication are provided.
Molecular and Cellular BiologyEvidence that replication initiates at only some of the potential origins in each oligomeric form of bovine papillomavirus type 1 DNA.
52 Citations1990Jorge B. Schvartzman, S. Adolph +2 more
Journal of VirologyThe product of the bovine papillomavirus type 1 modulator gene (M) is a phosphoprotein
38 Citations1988Lauren Thorner, Nathan Bucay +2 more
Journal of VirologyBovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity
37 Citations1990David J. Riese, J Settleman +2 more
Results indicate that the wild-type bovine papillomavirus type 1 genome encodes an E2 repressor protein that moderates the viral transforming activity and allows maintenance of the viral DNA at a relatively low copy number.
Journal of VirologyProteins encoded by the bovine papillomavirus E1 open reading frame: expression in heterologous systems and in virally transformed cells
36 Citations1990S. Santucci, Elliot J. Androphy +2 more
The capacity of the whole E1 ORF to encode a 72-kDa protein was demonstrated by translation of synthetic RNA in a reticulocyte lysate system, by microinjection of RNA into Xenopus oocytes, and by expression in recombinant baculoviruses and vaccinia viruses.
Journal of VirologyInduction of bovine papillomavirus E2 gene expression and early region transcription by cell growth arrest: correlation with viral DNA amplification and evidence for differential promoter induction
27 Citations1990S Burnett, Ann-Charlotte Ström +6 more
Observations indicate a central role for E2 transcription factors in the induction of viral DNA amplification in division-arrested cells in vitro and in vivo and suggest that this process is associated with a qualitative switch in the expression of viral early region genes.
Molecular and Cellular BiologyEvidence that Replication Initiates at Only Some of the Potential Origins in Each Oligomeric Form of Bovine Papillomavirus Type 1 DNA
26 Citations1990Jorge B. Schvartzman, S. Adolph +2 more
It is concluded that initiation of DNA replication in BPV-1 DNA takes place at or close to plasmid maintenance sequence 1.
Novartis Foundation symposiumThe Bovine Papillomavirus Replicon
26 Citations2007Michael R. Botchan, Leslie J. Berg +2 more
It is suggested that either the cell or the virus encodes a repressor, and cells harbouring plasmids with mutations in either the E6 or the E7 open reading frame acquire an immunity to high copy-number plasmid replication.
Journal of VirologyLoss of bovine papillomavirus DNA replication control in growth-arrested transformed cells
16 Citations1989S Burnett, U. Kießling +1 more
It appears that this process represents a mechanism employed by the virus to ensure maximal viral DNA synthesis within a growth-arrested cell.