Performance of the OraQuick® and Hema-Strip® rapid HIV antibody detection assays by non-laboratorians

Journal of Clinical VirologyPublished 5 February 2004

Timothy C. Granade, Bharat Parekh, Susan Phillips, J S McDougal

DOI10.1016/j.jcv.2003.12.006

Source URL

Citations44

SJR quartileQ1

SJR score1.19

SNIP0.90

TL;DR

Assessment of the ability of 99 individuals with no laboratory experience to conduct two RT, OraQuick and Hema-Strip, found the number of invalid tests was greatly reduced when participants received a demonstration.

Abstract

Rapid HIV antibody tests (RT) now permit HIV screening in settings where laboratory personnel may not be available. This study assessed the ability of 99 individuals with no laboratory experience to conduct two RT, OraQuick and Hema-Strip; these results were compared with those generated by laboratory professionals. All participants received written instructions and one-half also received a short demonstration. Error rates ranged from 2.1% to 4.6% with or without a demonstration. However, the number of invalid tests was greatly reduced when participants received a demonstration. Appropriate RT training for non-laboratorians and continued monitoring of HIV RT performance in non-laboratory settings is recommended.

Keywords

Immunology and MicrobiologyMedicine

AIDS Research and Human RetrovirusesQuantitative Detection of Increasing HIV Type 1 Antibodies after Seroconversion: A Simple Assay for Detecting Recent HIV Infection and Estimating Incidence

357 Citations2002Bharat Parekh, Melanie S. Kennedy +10 more

A simple enzyme immunoassay (EIA) that detects increasing levels of anti-HIV IgG after seroconversion and can be used for detecting recent HIV-1 infection and for estimating incidence among diverse HIV- 1 subtypes worldwide.

AIDSOn-site, rapid HIV testing with same-day results and counseling

162 Citations1997William J. Kassler, Beth Dillon +3 more

Rapid, on‐site HIV testing was feasible, preferred by clients, and resulted in significant improvement in the number of persons learning their serostatus, without increasing the costs or decreasing the effectiveness of counseling and testing.

Clinical ChemistryInstruments for Self-Monitoring of Blood Glucose: Comparisons of Testing Quality Achieved by Patients and a Technician

131 Citations2002Svein Skeie, Geir Thue +2 more

The analytical quality of SMBG among patients was poorer than, and could not be predicted from, the performance of the meters in the hands of a technician, and it is suggested that new instruments be tested in thehands of patients who are trained on meter use in a routine way.

View PDF

International Journal of STD & AIDSRapid HIV testing with same-day results: a field trial in Uganda

84 Citations1998William J. Kassler, Mary Grace Alwano-Edyegu +4 more

It is concluded that simple, rapid HIV tests can be performed accurately on-site within the time frame of a clinic visit, increasing the number of clients who learn their serostatus and receive post-test counselling.

Rapid Tests for HIV Antibody

74 Citations2000Bernard M. Branson

Rapid HIV tests are widely used in resource-poor settings, especially in developing countries, and the need for immediate HIV test results to make treatment decisions and to assist with prevention strategies portends their increased use in developed countries as well.

EpidemiologyHome and Point-of-Care Pregnancy Tests: A Review of the Technology

42 Citations2002Joel Ehrenkranz

Home pregnancy tests, which use monoclonal antibodies and visual labels in an immunochromatographic format, were introduced in the 1990s, and are able to reliably indicate pregnancy as early as 15 days after ovulation or 1 day after a missed menstrual period.

Clinical ChemistryImproving Results from Home Blood-Glucose Monitoring: Accuracy and Reliability Require Greater Patient Education as Well as Improved Technology

9 Citations2002M. Nattrass

Clinicians often sit in their offices or surgeries and wonder why the home blood-glucose monitoring record prepared by the patient is at odds with the objective measurement of control given by hemoglobin A1c, according to Skeie et al. (1).

View PDF