Microculture and Electrofusion of Defined Protoplasts of the Moss <i>Funaria hygrometrica</i>
Botanica ActaPublished 1 May 1988
Antonio Mejía, G. Spangenberg, H. -U. Koop, Martin Bopp
Citations19
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TL;DR
Electrofusion in different combinations of defined protoplast pairs of wild type and mutated forms produced products derived from one-to-one electric field induced fusion that were efficiently microcultured and somatic hybrids were regenerated.
Abstract
Abstract Protoplasts isolated from protonemata of Funaria hygrometrica wild type and developmentally abnormal mutants were individually selected and cultured in microdroplets of defined, unconditioned culture medium. Up to 60 % of the microcultured wild type protoplasts regenerated whole plants after 4 weeks. Electrofusion in different combinations of defined protoplast pairs of wild type and mutated forms was performed. The products derived from one‐to‐one electric field induced fusion were efficiently microcultured and somatic hybrids were regenerated.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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