Three possible disulfides in the acetylcholine receptor alpha-subunit.
Journal of Biological ChemistryPublished 1 January 1988Open access
R Mosckovitz, Jonathan M. Gershoni
Citations54
SJR quartileQ1
SJR score1.71
SNIP1.00
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TL;DR
The cysteinyl residues of the acetylcholine receptor alpha-subunit of Torpedo californica were analyzed and three possible disulfide bridges and one unpaired cysteine were indicated.
Abstract
The cysteinyl residues of the acetylcholine receptor alpha-subunit of Torpedo californica were analyzed. All seven cysteines could be accounted for. Three possible disulfide bridges and one unpaired cysteine were indicated. The disulfide linkages were as follows: Cys128 to Cys142; Cys192 to Cys193; Cys412 to Cys418 (Cys222 is unpaired). The identification of cysteinyl residues was accomplished by a modified protein blot procedure. Cysteinyl residues of intact nicotinic acetylcholine receptor were selectively biotinylated with 3-(N-maleimidopropionyl)biocytin and subsequently detected by the 125I-labeled avidin overlay of blotted Staphylococcus aureus V8 proteolyzed alpha-subunits. Two pairs of cysteines (Cys128/Cys142 and Cys412/Cys418) could be demonstrated only after Na(BH4) reduction of the acetylcholine receptor. Cysteine residues 192 and 193 are particularly sensitive to reduction; 0.1 mM dithiothreitol is sufficient.
Keywords
Biochemistry, Genetics and Molecular Biology
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