Characterization of a 3′ → 5′ exonuclease activity in the phage φ29-encoded DNA polymerase
Nucleic Acids ResearchPublished 1 January 1985Open access
Luis Blanco, Margarita Salas
Citations72
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TL;DR
The 3'----5' exonuclease activity was shown to be associated with the DNA polymerase since 1) the two activities were heat-inactivated with identical kinetics and 2) both activities, present in purified protein p2, cosedimented in a glycerol gradient.
Abstract
Purified protein p2 of phage phi 29, characterized as a specific DNA polymerase involved in the initiation and elongation of phi 29 DNA replication, contains a 3'----5' exonuclease active on single-stranded DNA, but not on double-stranded DNA. No 5'----3' exonuclease activity was found. The 3'----5' exonuclease activity was shown to be associated with the DNA polymerase since 1) the two activities were heat-inactivated with identical kinetics and 2) both activities, present in purified protein p2, cosedimented in a glycerol gradient.
Keywords
Biochemistry, Genetics and Molecular Biology
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