An in vitro slice preparation of the cat lateral geniculate nucleus
Journal of Neuroscience MethodsPublished 1 July 1987
Vincenzo Crunelli, Nathalie Leresche, J. Hynd, Naresh Patel, John G. Parnavelas
Citations19
SJR quartileQ3
SJR score0.73
SNIP0.87
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TL;DR
A slice preparation of the cat thalamus containing the lateral geniculate nucleus and the terminal portion of the optic tract is described, and intrasomatic injection of horseradish peroxidase used for the morphological identification of recorded neurones as X or Y cells.
Abstract
A slice preparation of the cat thalamus containing the lateral geniculate nucleus and the terminal portion of the optic tract is described. Ultrastructurally the slices remain relatively normal for only a short time after cutting. Indeed most cellular elements deteriorate quickly with time but patches of relatively intact tissue were still present even 10 h after cutting and maintenance in a storage bath. However, for 4-5 h after cutting long-lasting intracellular recordings of high quality and stability were obtained, and intrasomatic injection of horseradish peroxidase used for the morphological identification of recorded neurones as X or Y cells.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
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Using an in vitro preparation of the cat dorsal lateral geniculate nucleus, the passive membrane properties and the electrotonic structure of single cells each identified as X or Y on the basis of their morphological features following intrasomatic injection of horseradish peroxidase are studied.