Large‐Scale BAC Clone Restriction Digest Fingerprinting
Current Protocols in Human GeneticsPublished 1 April 2007
Carrie Mathewson, Jacqueline E. Schein, Marco A. Marra
Citations8
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TL;DR
This unit describes a robust, large‐scale procedure for generation of agarose gel–based clone fingerprints from BAC clones.
Abstract
Restriction digest fingerprinting is a common method for characterizing large insert genomic clones, e.g., bacterial artificial chromosome (BAC), P1 artificial chromosome (PAC) and Fosmid clones. This clone fingerprinting method has been widely applied in the construction of clone-based physical maps, which have been used as positional cloning resources as well as to support directed and genome-wide sequencing efforts. This unit describes a robust, large-scale procedure for generation of agarose gel-based clone fingerprints from BAC clones.
Keywords
Computer ScienceBiochemistry, Genetics and Molecular Biology
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