12‐<i>O</i>‐Tetradecanoylphorbol 13‐acetate stimulates inositol lipid phosphorylation in intact human platelets
FEBS LettersPublished 6 August 1984
Didier de Chaffoy de Courcelles, Peter Roevens, Herman Van Belle
Citations99
SJR quartileQ1
SJR score1.22
SNIP0.77
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TL;DR
Potyphosphoinositide Phorbol ester Protein kinase C tetrahydrolysis-like activity is reported at elevated levels in the presence of Na6(CO3)(SO4)2, Na2SO4, and Na2CO3 at low temperatures through the stationary phase.
Abstract
The phorbol esters are among the most potent tumor promoters. On addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to isolated human platelets prelabelled with [32P]orthophosphate we found a rapid increase in 32P incorporation into phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. In view of similar findings with cells infected with the oncogene Rous sarcoma virus, it is suggested that inositol lipid phosphorylation might be a key event in the molecular action of phorbol esters.
Keywords
Medicine
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