Cloning of the gene coding for human L apoferritin
Nucleic Acids ResearchPublished 1 January 1986Open access
Claudio Santoro, Maria Marone, Marina Ferrone, Francesco Costanzo, Maurizio Colombo, Carlo Minganti
Citations103
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Data is reported suggesting that a single gene is mainly expressed in several tissues examined, and its sequence shows three introns that are identical to that of cDNA clone isolated from human liver.
Abstract
A recently reported cDNA clone coding for human promyelocytic L apoferritin, shows some differences with a liver L apoferritin cDNA. We have investigated if these differences are due to the expression of different genes or to an alternative transcription of an unique gene. In this paper we report data suggesting that a single gene is mainly expressed in several tissues examined. This gene has been cloned and characterized. Its sequence shows three introns: the exon sequence is identical to that of cDNA clone isolated from human liver. A minimum of five related pseudogenes have been also analysed. One of them is a processed pseudogene interrupted by an intron-like fragment.
Keywords
Biochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesDNA sequencing with chain-terminating inhibitors
69,306 Citations1977Frederick Sanger, S. Nicklen +1 more
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
CellSizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
3,010 Citations1977Arnold Berk, Phillip A. Sharp
A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
GeneConstruction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
2,537 Citations1983Jan M. Norrander, Tomas Kempe +1 more
In adding the restriction endonuclease cleavage sites for SphI and KpnI to the lac cloning region of the phage vectors, this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes.
Journal of Molecular BiologyLambda replacement vectors carrying polylinker sequences
1,753 Citations1983Anna‐Maria Frischauf, Hans Lehrach +2 more
A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.
GeneThe making of strand-specific M13 probes
648 Citations1982Nien-Tai Hu, Joachim Messing
A novel approach has been developed for the preparation of highly radioactive, strand-specific M13 probes by using a universal primer to initiate the DNA synthesis of the complementary strand of the M13 sequence downstream from the inserted sequence.
Philosophical transactions of the Royal Society of London. Series B, Biological sciencesFerritin: design and formation of an iron-storage molecule
566 Citations1984Geoffrey C. Ford, P. M. Harrison +5 more
The structure of horse spleen apoferritin, which has recently been refined, consists of 24 symmetrically related subunits forming a near-spherical hollow shell, and the protein influences both the rate of FeII-oxidation and the form of oxide produced.
Journal of Biological ChemistryOn ferritin heterogeneity. Further evidence for heteropolymers.
464 Citations1978Paolo Arosio, T.G. Adelman +1 more
Results substantiate the proposal that tissue ferritins are not unique homopolymers but families of hybrid molecules consisting of different proportions of two subunit types and that both are species-specific.
CellA map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids
309 Citations1977Louise T. Chow, James M. Roberts +2 more
Using electron microscopic RNA loop mapping to determine, to within 200 nucleotides, the chromosome coordinates of the 5′ and 3′ ends of adenovirus type 2 transcripts isolated from the cytoplasm of productively infected human KB cells, the most frequent late RNA loop occurred between coordinates 51.9 and 62.8.
NatureElectron density map of apoferritin at 2.8-Å resolution
263 Citations1978Stephen Banyard, D.K. Stammers +1 more
The 6-Å resolution structure of apoferritin3 showed the presence of several rods of electron density, tentatively assigned as α helices, and channels passing through the shell, which could provide an access route for Fe atoms, and these features have been confirmed at 2.8-™ resolution and can now also provide a plausible subunit conformation and quaternary structure.
NatureMethylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites
247 Citations1978Bruno Gronenborn, Joachim Messing
A way of generating restriction endonuclease cleavage sites by single base changes derived after in vitro methylation of single-stranded DNA is described.
Journal of Biological ChemistryStructural and functional relationships of human ferritin H and L chains deduced from cDNA clones.
241 Citations1985Dana Boyd, Cecilia Vecoli +3 more
The first comparison of H and L nucleotide and amino acid sequences from the same species as well as ferritin L cDNA sequences from different species concludes that human H andL ferritins are related proteins which diverged about the time of evolution of birds and mammals.
The Journal of Experimental MedicineIdentification of leukemia-associated inhibitory activity as acidic isoferritins. A regulatory role for acidic isoferritins in the production of granulocytes and macrophages.
188 Citations1981Hal E. Broxmeyer, John Bognacki +2 more
Results implicate acidic isoferritins in the regulation of normal myelopoiesis and suggest a role for them in the progression of leukemia.
CellCommon and interchangeable elements in the promoters of genes transcribed by RNA polymerase III
174 Citations1983Gennaro Ciliberto, Giovanni Raugei +3 more
It is shown that the 34 bp internal control region of the somatic 5S RNA gene from Xenopus borealis can be split into two separable components.
British Journal of HaematologySuppressive Effect of Ferritin on <i>in Vitro</i> Lymphocyte Function
160 Citations1979Yaacov Matzner, Chaim Hershko +3 more
Ferritin suppresses certain parameters of T‐lymphocyte function in vitro, and the relation of the present findings to recognized abnormalities of T-cell function encountered in certain neoplastic disorders associated with high serum ferritin levels is at present unknown.
Proceedings of the National Academy of SciencesSelective isolation of cosmid clones by homologous recombination in Escherichia coli.
150 Citations1984Annemarie Poustka, Hans‐Richard Rackwitz +3 more
A procedure for selection of specific cosmid clones by homologous recombination between Cosmid clones from a library and sequences cloned into a plasmid vector without sequence homology to the cosmid vector has been developed.
The EMBO JournalStructure and expression of the human haptoglobin locus.
146 Citations1985G. Bensi, Giovanni Raugei +2 more
Northern blot and primer elongation analyses with human liver RNA show that the haptoglobin gene Hp1F appears to be transcribed some 1000‐fold less in fetal than in adult liver.
Journal of Biological ChemistryAdaptive responses of rat tissue isoferritins to iron administration. Changes in subunit synthesis, isoferritin abundance, and capacity for iron storage.
129 Citations1981Adrian Bomford, Christine Conlon-Hollingshead +1 more
Using 2-h pulse doses of [3H]leucine, it was shown that ferritin protein synthesis was maximally stimulated 12 to 18 h after iron administration, with a preferential increase in the proportion of new L sub units, thus favoring the assembly of isoferritins rich in L subunits.
Proceedings of the National Academy of SciencesPhorbol ester induction of leukemic cell differentiation is a membrane-mediated process.
86 Citations1982Richard A. Cooper, A D Braunwald +1 more
It is demonstrated that phorbol esters exert their effects while retained at the cell surface through the stimulation of phosphatidylcholine synthesis, which appears to be a part of a receptor-mediated, transmembrane process.
PubMedCloning of nematode tRNA genes and their expression in the frog oocyte.
78 Citations1978R. Cortese, Douglas A. Melton +2 more
Evidence is presented which suggests that these nematode tRNA genes are accurately transcribed and processed in frog oocytes and shows that cloned eukaryotic DNAs from a heterologous source can be tested for functional gene activity in X. laevis oocytes.
Journal of Biological ChemistryDifferential turnover of rat liver isoferritins.
76 Citations1980Yutaka Kohgo, M. Yokota +1 more
Rat liver ferritins consist of families of isoferritins fashioned from different proportions of two subunit types, H and L, and iron administration alters both the absolute and the relative amounts of these isoferrite through an increase in L-rich forms.
Proceedings of the National Academy of SciencesTranscription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
67 Citations1980R. Cortese, Richard M. Harland +1 more
It is concluded that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocyte nuclei and tRNA transcription is not observed when DNA synthesis is inhibited.
Proceedings of the National Academy of SciencesStructure of human ferritin light subunit messenger RNA: comparison with heavy subunit message and functional implications.
51 Citations1985M. Dörner, Jochen Salfeld +4 more
The common structural features of the H and L subunits lead us to conclude that they have diverged from a single ancestral gene.
GeneThe isolation of DNA from agarose gels by electrophoretic elution onto malachite green-polyacrylamide columns
49 Citations1978Barbara Koller, Hajo Delius +2 more
Electrophoretic elution of DNA coupled with direct adsorption onto malachite green-polyacrylamide columns was used to isolate double- and single-stranded DNA from agarose gels and heteroduplexes formed from the isolated complementary single strands of T7 wild type DNA and a T7 deletion mutant were examined.
FEBS LettersThe amino acid sequence of human liver apoferritin
36 Citations1983J.M. Addison, J.E. Fitton +3 more
The protein component of the iron storage molecule, ferritin, contains 24 subunits in form of a hollow shell known as apoferritin that shows extensive homology with the primary structures of apoferriteins from human and horse spleen and from rat liver.