Diagnostic tools for filariasis elimination programs
Trends in ParasitologyPublished 15 December 2006
Gary J. Weil, Reda M. R. Ramzy
Citations232
SJR quartileQ1
SJR score1.31
SNIP1.79
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TL;DR
This article reviews available diagnostic tests for filariasis and their potential use as tools for different phases of filARIasis elimination programs.
Abstract
The ambitious and exciting Global Programme to Eliminate Lymphatic Filariasis (GPELF) is largely based on a strategy of mass drug administration (MDA) of repeated rounds of antifilarial medications to endemic populations around the world. Diagnostic tools are important to GPELF because they affect decisions regarding where to distribute MDA, how to measure its effects, how to define targets and endpoints for stopping MDA, and how to monitor populations for possible resurgence of filariasis transmission following suspension of MDA. This article reviews available diagnostic tests for filariasis and their potential use as tools for different phases of filariasis elimination programs.
Keywords
MedicineEnvironmental Science
PubMedStrategies and tools for the control/elimination of lymphatic filariasis.
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441 Citations1997Gary J. Weil, Patrick J. Lammie +1 more
The authors found that the ICT card test was very easy to perform and that it was comparable with ELISA for the detection of filarial antigen in sera from people with microfilaremia.
Advances in Parasitology/Advances in parasitologyLymphatic Filariasis: Treatment, Control and Elimination
253 Citations2006Eric A. Ottesen
A public health dimension with a focus on affected populations, now supplements the earlier, predominantly patient-oriented clinical approach to LF, and the early uncertainties, then the essential steps leading to this change in outlook are outlined.
Transactions of the Royal Society of Tropical Medicine and HygieneEfficacy of single dose combinations of albendazole, ivermectin and diethylcarbamazine for the treatment of bancroftian filariasis
208 Citations1998M. M. Ismail, R. L. Jayakody +10 more
Alb/iver is a safe and effective single dose regimen for suppression of microfilaraemia in bancroftian filariasis that could be considered for control programmes and potential relative safety in areas of Africa where DEC cannot be used for Filariasis control.
PubMedA highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.
199 Citations1990Simon J. More, D. B. Copeman
A weak but significant correlation was found between numbers of microfilariae in blood and detectable levels of circulating antigen from patients with bancroftian filariasis, and a monoclonal antibody directed against antigens of Onchocerca gibsoni has been used in a sandwich ELISA.
The LancetEffect of yearly mass drug administration with diethylcarbamazine and albendazole on bancroftian filariasis in Egypt: a comprehensive assessment
187 Citations2006Reda M. R. Ramzy, M. El Setouhy +6 more
It is suggested that after five rounds of MDA filariasis is likely to have been eliminated in most endemic localities in Egypt.
American Journal of Tropical Medicine and HygieneA REAL-TIME PCR-BASED ASSAY FOR DETECTION OF WUCHERERIA BANCROFTI DNA IN BLOOD AND MOSQUITOES
160 Citations2006Ramakrishna U. Rao, LAURA J. ATKINSON +8 more
Real-time PCR has great potential as a tool for monitoring progress in large-scale filariasis elimination programs because of its high-throughput capacity and decreased risk of cross-contamination between test samples.
The Journal of Infectious DiseasesA Monoclonal Antibody-Based Enzyme Immunoassay for Detecting Parasite Antigenemia in Bancroftian Filariasis
148 Citations1987Gary J. Weil, D. C. Jain +6 more
Investigating parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W. bancrofti infection and may be explained in part by antibody-mediated clearance of circulating antigen.
Filaria JournalRecombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis – a multicenter trial
142 Citations2004Patrick J. Lammie, Gary J. Weil +6 more
Well-designed field studies are now needed to assess sampling methodology and the application of antibody testing to the monitoring and surveillance of LF elimination programs, and the performance of antibody assays for filariasis based on recombinant antigens Bm14, WbSXP, and BmR1.
Annals of Tropical Medicine and ParasitologyThe use of spatial analysis in mapping the distribution of bancroftian filariasis in four West African countries
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The results indicate that bancroftian filariasis is more widely distributed in arid areas of Burkina Faso than hitherto recognized and that the prevalences of infection have remained fairly stable for at least 30 years.
Transactions of the Royal Society of Tropical Medicine and HygieneA polymerase chain reaction-based assay for detection of Wuchereria bancrofti in human blood and Culex pipiens
106 Citations1997Reda M. R. Ramzy, Hoda A. Farid +7 more
PCR is a powerful epidemiological tool for screening villages for the prevalence of W. bancrofti and could be used initially to locate endemic areas with transmission of bANCroftian filariasis.
American Journal of Tropical Medicine and HygieneIMPORTANT EXPERIMENTAL PARAMETERS FOR DETERMINING INFECTION RATES IN ARTHROPOD VECTORS USING POOL SCREENING APPROACHES
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It is shown that, under most conditions in which one would want to use group testing, most of the error results from sampling and not the pooling process, and the meaning of confidence intervals associated with prevalence estimates and the appropriate interpretation of these intervals are discussed.
The Journal of ImmunologyMonoclonal antibodies to parasite antigens found in the serum of <i>Dirofilaria immitis</i>-infected dogs.
102 Citations1985Gary J. Weil, Michelle S. Malane +2 more
Parasite antigen detection with this monoclonal antibody-based ELISA appears to be superior to previously described diagnostic methods for canine dirofilariasis in terms of sensitivity, specificity, and relation to infection intensity.
Trends in ParasitologyMathematical models and lymphatic filariasis control: endpoints and optimal interventions
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This work states that mathematical models of filariasis transmission can serve as an effective tool for guiding the scientific development and management of successful community-level intervention programmes by acting as analytical frameworks for integrating knowledge regarding parasite transmission dynamics with programmatic factors.
American Journal of Tropical Medicine and HygieneA longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.
82 Citations1999Gary J. Weil, Reda M. R. Ramzy +4 more
It is suggested that humans develop partial immunity to Wuchereria bancrofti over time, and incident infections approximately balanced infections lost to produce an apparent state of dynamic equilibrium.
PubMedA comparison of the Og4C3 antigen capture ELISA, the Knott test, an IgG4 assay and clinical signs, in the diagnosis of Bancroftian filariasis.
82 Citations1993Peter Turner, B. Copeman +2 more
Concern is raised over the underestimation of the prevalence of filariasis due to ineffective diagnostic criteria and the two ELISA tests should be of value in epidemiological surveys and for monitoring Filariasis control programs.
Molecular and Biochemical ParasitologyMolecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis
80 Citations1994Ramaswamy Chandrashekar, Kurt C. Curtis +4 more
Assays based on two filaria-specific recombinant clones with immunodiagnostic potential may be useful for large scale screening as an alternative to microfilaria or filarial antigen detection as a means of obtaining a rough index of filariasis endemicity in previously unstudied areas.
American Journal of Tropical Medicine and HygieneParasite Antigenemia without Microfilaremia in Bancroftian Filariasis
77 Citations1996Gary J. Weil, Reda M. R. Ramzy +4 more
Evidence is reported that amicrofilaremic and asymptomatic W. bancrofti infections are relatively common in endemic areas and additional studies are needed to determine the clinical significance, prognosis, and optimal management of such infections.
The Journal of Infectious DiseasesClearance of Circulating Filarial Antigen as a Measure of the Macrofilaricidal Activity of Diethylcarbamazine in Wuchereria bancrofti Infection
71 Citations1995James McCarthy, A. Guinea +2 more
The complete clearance of CFA observed after therapy with DEC indicates that assessment of C FA clearance is a useful means for detecting macrofilaricidal effects of antifilarial chemotherapy.
Annals of Tropical Medicine and ParasitologyDevelopment and standardization of a rapid, PCR-based method for the detection of <I>Wuchereria bancrofti</I> in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis
68 Citations2002Steven A. Williams, Sandra J. Laney +12 more
The first round of testing was designed to test the four most promising methods for DNA extraction from pools of mosquitoes and two of the four methods stood out as clearly the best and these will be now optimised and evaluated in two further rounds of testing.
The Journal of ImmunologyThe Serpin Secreted by <i>Brugia malayi</i> Microfilariae, Bm-SPN-2, Elicits Strong, but Short-Lived, Immune Responses in Mice and Humans
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It is demonstrated that Bm-SPN-2 is recognized by both murine and human T and B cells and indicate that their responses are under relatively stringent temporal control, providing the first example of a stage-specific secreted molecule that acts as a major T cell Ag from filarial parasites and is a prime candidate for a serodiagnostic probe.
Transactions of the Royal Society of Tropical Medicine and HygieneSpecificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection
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The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value, and Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection.
Journal of Clinical MicrobiologyDetection of <i>Brugia</i> Parasite DNA in Human Blood by Real-Time PCR
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The Journal of ImmunologyIdentification and partial characterization of a parasite antigen in sera from humans infected with <i>Wuchereria bancrofti</i>.
66 Citations1987Gary J. Weil, Fanya Liftis
The purpose of this study was to identify and characterize soluble parasite antigens present in sera from humans infected with the filarial nematode Wuchereria bancrofti, and its presence in human serum appears to be specific for W. banc rofti infection.
Transactions of the Royal Society of Tropical Medicine and HygieneLong-term efficacy of single-dose combinations of albendazole, ivermectin and diethylcarbamazine for the treatment of bancroftian filariasis
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American Journal of Tropical Medicine and HygieneA RANDOMIZED CLINICAL TRIAL COMPARING SINGLE- AND MULTI-DOSE COMBINATION THERAPY WITH DIETHYLCARBAMAZINE AND ALBENDAZOLE FOR TREATMENT OF BANCROFTIAN FILARIASIS
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Transactions of the Royal Society of Tropical Medicine and HygieneBancroftian filariasis: effect of repeated treatment with diethylcarbamazine and albendazole on microfilaraemia, antigenaemia and antifilarial antibodies
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American Journal of Tropical Medicine and HygieneBancroftian filariasis in Egypt: visualization of adult worms and subclinical lymphatic pathology by scrotal ultrasound.
52 Citations1998R Faris, Omar Hussain +3 more
The value of scrotal ultrasound as a means of noninvasively visualizing adult filarial worms and assessing subclinical lymphatic damage in Bancroftian filariasis is confirmed.
Molecular and Biochemical ParasitologyA cloned antigen for serological diagnosis of Wuchereria bancrofti microfilaremia with daytime blood samples
49 Citations1992Senarath Dissanayake, Min Xu +1 more
These findings indicate that it may be possible to replace microscopic examination of night blood films with a serological test designed to detect antibodies to a mix of SXP-1 and other suitable antigens for the diagnosis of microfilaremia due to bancroftian filariasis.
American Journal of Tropical Medicine and HygieneHyperendemic Bancroftian Filariasis in the Kingdom of Tonga: The Application of the Membrane Filter Concentration Technique to an Age-Stratified Blood Survey
48 Citations1974Robert S. Desowitz, James C. Hitchcock
A survey carried out in Tonga compared the diagnostic efficiency of the membrane-filtration and stained blood film techniques, which revealed the prsence of microfilariae in the blood of 5 of 8 patients with gross elephantiasis, whereas microFilariae were found in the stained thick-film of only 1 of these individuals.
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47 Citations2003Jeevan B. Sherchand, Valérie Obsomer +2 more
The study indicates that the prevalence of infection by Wuchereria bancrofti is far greater that was previously reported and that lymphatic filariasis should be a much higher health priority than currently given.
American Journal of Tropical Medicine and HygieneSensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples.
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American Journal of Tropical Medicine and HygienePersistence of Parasite Antigenemia Following Diethylcarbamazine Therapy of Bancroftian Filariasis
40 Citations1988Gary J. Weil, Kandan Sethumadhavan +3 more
The results suggest that diethylcarbamazine has only partial macrofilaricidal activity against W. bancrofti with this dosage schedule and that parasite antigen detection represents a valuable new approach for monitoring the efficacy of antifilarial drug therapy.
Eastern Mediterranean Health JournalField evaluation of a rapid-format kit for the diagnosis of bancroftian filariasis in Egypt
38 Citations1999Reda M. R. Ramzy, H. Helmy +2 more
The AMRAD-ICT Filariasis Test (ICT-Fil) is a new, rapid-format card test for the detection of bancroftian antigenaemia in human blood evaluated under field conditions in Egypt by comparing 1813 endemic and 102 nonendemic participants.
Annals of Tropical Medicine and ParasitologyEvaluation of a recombinant antigen-based antibody assay for diagnosis of bancroftian filariasis in Egypt
34 Citations1995Reda M. R. Ramzy, H. Helmy +4 more
Transactions of the Royal Society of Tropical Medicine and HygieneVector-borne infections in the tropics and health policy issues in the twenty-first century
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The challenge of implementing a 'vertical' approach to disease control within national health programmes, in the face of significant donor opposition to such programmes is a challenge even when such approaches will secure a 'public good'.
Filaria JournalAdvances and challenges in predicting the impact of lymphatic filariasis elimination programmes by mathematical modelling
34 Citations2006Wilma A. Stolk, Sake J. de Vlas +1 more
The two main challenges for future modelling work are quantification and validation of the models for other regions, for investigation of elimination prospects in situations with other vector-parasite combinations and endemicity levels than in Pondicherry.
Transactions of the Royal Society of Tropical Medicine and HygieneCommunity diagnosis of Bancroftian filariasis
31 Citations1993Rifky Faris, Reda M. R. Ramzy +3 more
A combination of clinical examination and the filarial antigen test (with optional examination for microfilariae of those with positive antigen tests) for community diagnosis of Bancroftian filariasis in endemic areas is recommended.
Transactions of the Royal Society of Tropical Medicine and HygieneInfluence of infection with non-filarial helminths on the specificity of serological assays for antifilarial immunoglobulin G4
29 Citations2003Andrew Muck, Maria Luíza Lemos Pires +1 more
The data suggest that serodiagnostic assays based on the use of crude filarial antigens should be interpreted with caution because of the potential for cross-reactivity with Strongyloides.
Tropical Medicine & International HealthField validation of sensitivity and specificity of rapid test for detection of <i>Brugia malayi</i> infection
28 Citations2005M. Jamail, Kashini Andrew +4 more
The rapid dipstick test was useful as a diagnostic tool for mapping and certification phases of the lymphatic filariasis elimination programme in B. malayi‐endemic areas.
Annals of Tropical Medicine and ParasitologyDetection of filaria-specific IgG<sub>4</sub>antibodies and filarial DNA, for the screening of blood spots for<i>Brugia timori</i>
27 Citations2005Peter Fischer, I. Bonow +3 more
The combined use of BR tests and PCR-based assays, for testing blood spots in areas where brugian filariasis is endemic, appears to be a promising method not only for post-treatment monitoring but also for the certification activities planned within the framework of the Global Programme to Eliminate Lymphatic Filariasis.
Journal of ParasitologyCirculating Parasite Antigen in Brugia pahangi-Infected Jirds
26 Citations1990Gary J. Weil, Ramaswamy Chandrashekar +4 more
It is concluded that parasite antigen detection allows B. pahangi development and survival as well as infection intensity to be monitored in living animals with unprecedented sensitivity and accuracy.
Tropical Medicine & International HealthTest strip detection of <i>Wuchereria bancrofti</i> amplified DNA in wild‐caught <i>Culex pipiens</i> and estimation of infection rate by a PoolScreen algorithm
24 Citations2004Hanan Helmy, Peter Fischer +3 more
Because it is faster and safer, the PCR test strip is a practical tool, especially when combined with the PoolScreen algorithm method, for xenomonitoring the success of elimination programmes.
Annals of Tropical Medicine and ParasitologyHuman infection with<i>Wuchereria bancrofti</i>in Matara, Sri Lanka: the use, in parallel, of an ELISA to detect filaria-specific IgG<sub>4</sub>in urine and of ICT card tests to detect filarial antigen in whole blood
18 Citations2003M. V. Weerasooriya, Makoto Itoh +5 more
Urine samples are easier, less painful and safer to collect than blood samples, and the ELISA may be particularly useful to screen very young and school-age children, to estimate current levels of transmission in a particular area.
Medical Microbiology and ImmunologyLymphatic filariasis in Papua New Guinea: prospects for elimination
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Filaria JournalHomologs of the Brugia malayi diagnostic antigen BmR1 are present in other filarial parasites but induce different humoral immune responses.
16 Citations2004Rahmah Noordin, Ros Azeana Abdul Aziz +1 more
The BmR1 antigen is suitable for detection of IgG4 antibodies in brugian filariasis, however, its homologs are not suitable for IgG 4-based diagnosis of other filarial infections.
Tropical Medicine & International HealthShort Communication: Observations on false positive reactions in the rapid NOW<sup>®</sup> Filariasis card test
13 Citations2004Paul E. Simonsen, Stephen Magesa
It was reaffirmed that test cards should be read after 10 min to avoid false positives, but it also indicated that experienced test card users should be able to make a correct diagnosis even at a later time.
PubMedStudies on the transmission potential of filariasis in controlled areas of Henan Province.
5 Citations1997Biao Xu, Z Cui +5 more
The results suggested that after the microfilarial rate was lower than 1%, the residual microfilaremias became negative gradually in 3-6 years, and the transmission of the disease was blocked.