Origin of Concatemeric T7DNA
Nature New BiologyPublished 1 October 1972
James D. Watson
Citations1,606
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TL;DR
The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails and can then join to form dimers which on further replication similarly form larger concatemer.
Abstract
The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails. Right-ended and left-ended molecules can then join to form dimers which on further replication similarly form larger concatemers.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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Heterozygotes formed by deletion ri mutants and r+, and by the host range alleles hI+ and h4±, were shown to behave as if they were due to this terminal redundancy of chromosomes of phage T4.
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T3 and T7 DNA molecules are terminally repetitious but non-permuted; T2 DNA is terminally repetition and permuted.
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It is shown that the rapidly-sedimenting form found in T7-infected cells is, in fact, T7 DNA, that it represents a true intermediate in the production of progeny T8 DNA molecules, and that it sediments more rapidly because it is longer than mature T7DNA.
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The gene 6 exonuclease is involved in the acid solubilization of bacterial DNA after T7 phage infection and may also be involved in other processes such as genetic recombination.
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It is concluded that newly replicated polynucleotide chains are chemically joined to chains of complementary sequence.
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The results of analyses of replicative and recombinant T7 DNA in both native and denatured form, as well as evidence for the participation of one or both strands in transfer to the progeny, are necessary to discriminate between the several possible ways in which a population of unique molecules may be generated.
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After infection of Escherichia coli B with radiolabeled T7 bacteriophage, the parental deoxyribonucleic acid label was found in both polynucleotide chains of the intracellular T7 concatemer.
Proceedings of the National Academy of SciencesTerminal Cross-Linking of DNA Strands by an Enzyme System from <i>Escherichia coli</i> Infected with Bacteriophage T4
12 Citations1970Bernard Weiss
An enzyme system, purified 560-fold from Escherichia coli infected with bacteriophage T4, catalyzes the formation of a phosphodiester bond between the original 5'-phosphoryl end-group of a DNA strand and a 3'-hydroxyl group of the complementary strand, resulting in a terminally cross-linked, spontaneously renaturable DNA duplex.
