C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression
Nature GeneticsPublished 7 April 2003
Jérôme Reboul, Philippe Vaglio, Jean‐François Rual, Philippe Lamesch, Monica Martinez, Christopher M. Armstrong
Citations388
SJR quartileQ1
SJR score16.59
SNIP6.64
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TL;DR
Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans successfully, and it is suggested that similar ORFeome projects will be valuable for other organisms, including humans.
Abstract
To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% of predicted genes needed corrections in their intron-exon structures. Notably, approximately 11,000 C. elegans proteins can now be expressed under many conditions and characterized using various high-throughput strategies, including large-scale interactome mapping. We suggest that similar ORFeome projects will be valuable for other organisms, including humans.
Keywords
Biochemistry, Genetics and Molecular Biology
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