In Vitro FRAP Identifies the Minimal Requirements for Mad2 Kinetochore Dynamics
Current BiologyPublished 1 April 2006Open access
Martin Vink, Marco Simonetta, Pietro Transidico, Karin Johanna Ferrari, Marina Mapelli, Anna De Antoni
Citations139
SJR quartileQ1
SJR score2.71
SNIP1.83
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TL;DR
In vitro FRAP is a widely applicable approach to dissecting the molecular bases of the interaction of a macromolecule with an insoluble cellular scaffold and the combination of in vitro fluorescence recovery after photobleaching with additional fluorescence-based assays in vitro can be used to unveil mechanism, stoichiometry, and kinetic parameters of amacromolecular interaction, all of which are important for modeling protein interaction networks.
Abstract
In vitro FRAP is a widely applicable approach to dissecting the molecular bases of the interaction of a macromolecule with an insoluble cellular scaffold. The combination of in vitro fluorescence recovery after photobleaching with additional fluorescence-based assays in vitro can be used to unveil mechanism, stoichiometry, and kinetic parameters of a macromolecular interaction, all of which are important for modeling protein interaction networks.
Keywords
Biochemistry, Genetics and Molecular Biology
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