Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.
Proceedings of the National Academy of SciencesPublished 1 December 1988Open access
D. Pinkel, J. E. Landegent, Colin C. Collins, James C. Fuscoe, Richard Segraves, J. N. Lucas
Citations1,340
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TL;DR
Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries to inhibit the hybridization of sequences in the library that bind to multiple chromosomes.
Abstract
Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries. Unlabeled human genomic DNA is used to inhibit the hybridization of sequences in the library that bind to multiple chromosomes. The target chromosome can be made at least 20 times brighter per unit length than the others. Trisomy 21 and translocations involving chromosome 4 can be detected in metaphase spreads and interphase nuclei by using this technique.
Keywords
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