A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases
Journal of VirologyPublished 1 July 1994Open access
A. Lorena Passarelli, Jason Todd, Lois K. Miller
Citations87
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TL;DR
A novel baculovirus gene, late expression factor eight gene (lef-8), of Autographa californica nuclear polyhedrosis virus is identified and sequenced that is necessary for efficient expression from late and very late virus gene promoters in a transient expression assay.
Abstract
We have identified and sequenced a novel baculovirus gene, late expression factor eight gene (lef-8), of Autographa californica nuclear polyhedrosis virus that is necessary for efficient expression from late and very late virus gene promoters in a transient expression assay. The predicted gene product, LEF-8, has a molecular mass of 102 kDa and contains a conserved sequence motif, GXKX4HGQ/NKG, found in DNA-directed RNA polymerases throughout the animal, plant, and microbial kingdoms.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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