Upstream Regions Directing Heart-Specific Expression of the GATA6 Gene During Mouse Early Development
The Journal of BiochemistryPublished 1 April 2000
Ge‐Hong Sun‐Wada, Sadao Manabe, Takao Yoshimizu, Chie Yamaguchi, Tatsufumi Oka, Yoh Wada
Citations12
SJR quartileQ2
SJR score0.79
SNIP0.59
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TL;DR
The 5' upstream region of the mouse GATA6 gene is characterized, and two closely spaced transcription initiation sites are identified, and the flanking sequence lacks a typical TATA-box, and is rich in guanine and cytosine.
Abstract
The expression of murine transcription factor GATA6 is restricted to tissues including the heart and gastrointestinal systems during embryogenesis, and is maintained throughout postnatal life. We have characterized the 5' upstream region (6.4 kb) of the mouse GATA6 gene, and identified two closely spaced transcription initiation sites. The flanking sequence lacks a typical TATA-box, and is rich in guanine and cytosine. The role of the 5' upstream region was examined using the lacZ reporter gene in transgenic mice. A construct containing the 5' flanking sequence (4.9 kb), untranslated exon 1 and 1.3 kb intron 1 could drive the gene expression in the embryonic and adult heart regions. Weak expression was also observed in the stomach, liver, and bronchial arch in addition to the cardiac region. Deletion of the 5' upstream region ( approximately 1.2 kb) or intron 1 abolished all this expression, indicating that at least two cis-acting control elements are necessary for heart-specific expression of GATA6 in vivo.
Keywords
Biochemistry, Genetics and Molecular Biology
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