Signalling Mechanisms Involved in the Induction of Inducible Nitric Oxide Synthase by Lactobacillus Rhamnosus GG, Endotoxin, and Lipoteichoic Acid
InflammationPublished 1 October 2002
Riku Korhonen, Riitta Korpela, Eeva Moilanen
Citations33
SJR quartileQ2
SJR score1.27
SNIP1.04
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TL;DR
Lactobacillus GG induced NO production through iNOS pathway and the mechanisms mediating that process were very similar with those involved in LPS and LTA induced NO synthesis.
Abstract
Lactobacillus GG induced NO production through iNOS pathway and the mechanisms mediating that process were very similar with those involved in LPS and LTA induced NO synthesis.
Keywords
Immunology and MicrobiologyAgricultural and Biological SciencesMedicine
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2,678 Citations2001W. Alderton, Chris E. Cooper +1 more
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2,221 Citations1994Q.W. Xie, Yumiko Kashiwabara +1 more
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Journal of Leukocyte BiologyEndotoxin signal transduction in macrophages
806 Citations1996Matthew J. Sweet, David Hume
The effects of LPS on macrophages are examined by following events from the cell surface to the nucleus and the involvement of protein tyrosine kinase, mitogen‐activated protein kinases, protein kinase C, G proteins, Protein kinase A, ceramide‐activatedprotein kinase and microtubules in this process are reviewed.
The FASEB JournalMolecular mechanisms and therapeutic strategies related to nitric oxide
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A brief overview will discuss some possible interventions in the L‐arginine:NO pathway and the potential benefits as well as undesirable side effects that might arise from them.
Pediatric ResearchEnhancement of the Circulating Antibody Secreting Cell Response in Human Diarrhea by a Human Lactobacillus Strain
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The results indicate that Lactobacillus GG promotes recovery from rotavirus diarrhea via augmentation of the local immune defense, which is possibly relevant in protection against reinfections.
PubMedA human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children.
691 Citations1991Erika Isolauri, Miia Juntunen +3 more
The result indicates that early nutritional repletion after rehydration causes no mucosal disruption and is beneficial for recovery from diarrhea, and it is further suggested that Lactobacillus GG in the form of fermented milk or freeze-dried powder is effective in shortening the course of acute diarrhea.
Antimicrobial Agents and ChemotherapyAntimicrobial substance from a human Lactobacillus strain
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Assessment of the role of the transcription factor NF-κB in both human liver (AKN-1) and human lung (A549) epithelial cell lines indicates that NF-σB activation is required for cytokine induction of the human iNOS gene and identifies four NF-β enhancer elements upstream in the humaniNOS promoter that confer inducibility to tumor necrosis factor-α and interleukin-1β.
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AP-1 and NF-κB are important cis-elements for induction of hiNOS gene transcription in cytokine-mediated induction of human inducible nitric oxide synthase (hiNOS) promoter activity.
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298 Citations1999Ching‐Chow Chen, James K. T. Wang
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Journal of Pediatric Gastroenterology and NutritionTreatment of Recurrent Clostridium difficile Colitis With Lactobacillus GG
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It is indicated that CsA and FK506 inhibit the induction of NOS, rather than its catalytic activity, which is unlikely to associated with a change in BH4 synthesis caused by these agents in LPS-treated J774 macrophages.
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FEBS LettersCoexpression of GTP cyclohydrolase I and inducible nitric oxide synthase mRNAs in mouse osteoblastic cells activated by proinflammatory cytokines
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32 Citations2000Lähde, Riku Korhonen +1 more
Intestinal epithelial cells express iNOS and produce nitric oxide in a nuclear factor-kappa B-dependent manner when exposed to LPS, implicated in the regulation of gut mucosal immunity.
Biochemical and Biophysical Research CommunicationsTetrahydrobiopterin Is a Secretory Product of Murine Vascular Endothelial Cells
27 Citations1994Roland B. Walter, Andreas Schaffner +3 more
In septic conditions, BH4 released by endothelial cells in large amounts could serve induced nitric oxide synthase in smooth muscle cells, thereby acting as another endothelium-derived relaxing factor mediating vasodilatation.
American Journal of Physiology-Gastrointestinal and Liver PhysiologyEndogenous but not exogenous nitric oxide decreases TNF-alpha-induced leukocyte rolling
22 Citations1997Paul Kubes, Elaine Sihota +1 more
The possibility of a physiological role for the increased production of NO from iNOS, i.e., regulation of leukocyte rolling and potentially the inflammatory response, is raised.